Identification of Campylobacter fetus by PCR-DNA probe method.

Journal of Clinical Microbiology
K BlomB Swaminathan

Abstract

A PCR method for rapid identification of Campylobacter fetus subsp. fetus was evaluated. A fragment of the gene coding for 16S rRNA was amplified from crude cell lysates of 18 C. fetus strains and 30 strains representing other Campylobacter species and subspecies. The amplicons were probed by dot blot hybridization with a digoxigenin-labeled C. fetus-specific oligonucleotide probe. The probe reacted only with C. fetus subsp. fetus and C. fetus subsp. venerealis and may be useful for rapid identification in clinical laboratories.

References

Sep 1, 1990·Journal of Clinical Microbiology·K H WilsonR C Greene
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W I WoodR M Lawn
Jun 1, 1993·Journal of Clinical Microbiology·I NachamkinC M Patton

❮ Previous
Next ❯

Citations

Dec 23, 2009·Memórias do Instituto Oswaldo Cruz·E M D ScheideggerP Cardarelli-Leite

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.