Identification of Campylobacter jejuni ATCC 43431-specific genes by whole microbial genome comparisons

Journal of Bacteriology
Frédéric PolyAlain Stintzi

Abstract

This study describes a novel approach to identify unique genomic DNA sequences from the unsequenced strain C. jejuni ATCC 43431 by comparison with the sequenced strain C. jejuni NCTC 11168. A shotgun DNA microarray was constructed by arraying 9,600 individual DNA fragments from a C. jejuni ATCC 43431 genomic library onto a glass slide. DNA fragments unique to C. jejuni ATCC 43431 were identified by competitive hybridization to the array with genomic DNA of C. jejuni NCTC 11168. The plasmids containing unique DNA fragments were sequenced, allowing the identification of up to 130 complete and incomplete genes. Potential biological roles were assigned to 66% of the unique open reading frames. The mean G+C content of these unique genes (26%) differs significantly from the G+C content of the entire C. jejuni genome (30.6%). This suggests that they may have been acquired through horizontal gene transfer from an organism with a G+C content lower than that of C. jejuni. Because the two C. jejuni strains differ by Penner serotype, a large proportion of the unique ATCC 43431 genes encode proteins involved in lipooligosaccharide and capsular biosynthesis, as expected. Several unique open reading frames encode enzymes which may contribute ...Continue Reading

References

Nov 1, 1992·Journal of Medical Microbiology·P H EverestP H Williams
Oct 1, 1989·Infection and Immunity·M E Konkel, L A Joens
Feb 1, 1985·The Journal of Hygiene·D G NewellA D Pearson
Dec 1, 1983·Infection and Immunity·M B CaldwellJ E Rogers
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·G SegalH A Shuman
Apr 14, 1999·Molecular Microbiology·C Whitfield, I S Roberts
Jul 19, 2000·Infection and Immunity·D J BaconP Guerry
Apr 28, 2001·Journal of Clinical Microbiology·E M RibotT J Barrett
Aug 22, 2001·Trends in Microbiology·D J KopeckoK J Zaal
Oct 16, 2002·Infection and Immunity·David J BaconPatricia Guerry
Nov 14, 2002·Genome Biology·Charles C KimStanley Falkow
Jun 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sebastian SuerbaumJames G Fox
Sep 2, 2003·Microbiology·Qun LiPeter R Reeves

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Citations

Oct 21, 2004·Nature Reviews. Microbiology·Mark EppingerStephan C Schuster
Nov 9, 2007·Journal of Clinical Neuromuscular Disease·Afshin Borhani Haghighi, Behnam Sabayan
Nov 29, 2007·Current Opinion in Gastroenterology·Frédéric Poly, Patricia Guerry
Apr 18, 2007·Infection and Immunity·Frédéric PolyPatricia Guerry
May 23, 2007·Infection and Immunity·Frédéric PolyPatricia Guerry
Jul 25, 2006·Infection and Immunity·Dirk HofreuterJorge E Galán
Aug 20, 2005·Journal of Bacteriology·Douwe MolenaarMichiel Kleerebezem
May 6, 2005·Journal of Clinical Microbiology·Frédéric PolyAlain Stintzi
Feb 10, 2007·BMC Evolutionary Biology·Pradeep Reddy MarriG Brian Golding
Oct 11, 2012·PloS One·Christina S VeggeHanne Ingmer
Aug 8, 2009·Future Microbiology·John M Atack, David J Kelly
Sep 18, 2007·The Journal of Molecular Diagnostics : JMD·Wenyong W Zhang, James Versalovic
Jun 19, 2008·Journal of Applied Microbiology·I HanningM Slavik
Jul 23, 2013·Journal of Microbiological Methods·Eduardo N TaboadaCatherine D Carrillo
Nov 6, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A van BelkumUNKNOWN European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group on Epidemiological Markers (ESGEM)
Jul 30, 2005·FEMS Immunology and Medical Microbiology·Kenta WatanabeMasahisa Watarai
May 2, 2007·Water Environment Research : a Research Publication of the Water Environment Federation·Dieter M TourlousseSyed A Hashsham
Apr 10, 2010·Journal of Food Protection·N HaddadJ-M Cappelier
Feb 10, 2021·Scientific Reports·Helena JuricovaIvan Rychlik

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