Identification of candidate biomarkers that involved in the epigenetic transcriptional regulation for detection gastric cancer by iTRAQ based quantitative proteomic analysis

Clinica Chimica Acta; International Journal of Clinical Chemistry
Zhen JiangLi Wang

Abstract

The sensitivities and specificities of biomarkers for gastric cancer are insufficient for clinical detection, and new diagnostics are therefore urgently required. A discovery set of gastric cancer tissues was labeled with iTRAQ reagents, separated using SCX chromatography, and identified using LC-ESI-MS/MS. A validation set of gastric cancer tissues was used to confirm the expression levels of potential markers. The present study detected metastasis-associated protein 2 (MTA2) and Histone deacetylases 1 (HDAC1) proteins that were overexpressed in gastric cancer tissues compared with that in adjacent gastric tissue. The sensitivity and specificity of MTA2 in detecting 76 cases gastric cancers were 57.9% (95% CI: 46.5%-69.3%) and 55.3% (95% CI: 43.8%-66.7%), respectively. The sensitivity and specificity of HDAC1 were 61.8% (95% CI: 50.7%-73%) and 63.2% (95% CI: 52.1%-74.3%), respectively. The co-expression of MTA2 and HDAC1 in gastric cancer achieved 65.3% sensitivity (95% CI: 51.5%-79.1%) and 65.2% specificity (95% CI: 50.9%-79.5%), which was strongly associated with lymph node metastasis and TNM staging. The present findings indicated a tight correlation between the MTA2 and HDAC1 expression level and lymph node metastasis and ...Continue Reading

References

Oct 12, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hidefumi SasakiYoshitaka Fujii
Mar 17, 2005·Breast Cancer Research and Treatment·Claudia A KruscheJoachim Alfer
May 10, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Farin KamangarWilliam F Anderson
Jul 11, 2006·European Journal of Gastroenterology & Hepatology·Matthias P A Ebert, Christoph Röcken
Sep 12, 2006·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yuxin JiDing Ma
Dec 1, 2005·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Hye-Rim ParkYong-Koo Park
Aug 31, 2010·Cytoskeleton·Mutsuki AmanoKozo Kaibuchi
Jan 5, 2011·Journal of Biomedicine & Biotechnology·Chiara V Segré, Susanna Chiocca
Apr 5, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Stamatios TheocharisXavier Sastre-Garau
Jan 31, 2012·Cellular and Molecular Life Sciences : CMLS·Nina ReichertPatrick Matthias
Feb 13, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Da-Wei ChenXiao-Xiao Jiang
May 24, 2013·Biochemical Society Transactions·Richard D W Kelly, Shaun M Cowley
Feb 26, 2014·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Claudio PelucchiEva Negri
Jul 17, 2014·Journal of Proteome Research·Alfonso Martín-BernabéSandrine Bourgoin-Voillard
Nov 16, 2014·Cancer Metastasis Reviews·Jian LiuHaili Qian
Jun 13, 2015·Disease Markers·Jianhong WuJianjun Wang
Feb 13, 2016·Gene·Rakesh Kumar, Rui-An Wang
Nov 26, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Zhen JiangLi Wang

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.