Identification of canine platelet proteins separated by differential detergent fractionation for nonelectrophoretic proteomics analyzed by Gene Ontology and pathways analysis

Veterinary Medicine
Shauna A TrichlerCamilo Bulla

Abstract

During platelet development, proteins necessary for the many functional roles of the platelet are stored within cytoplasmic granules. Platelets have also been shown to take up and store many plasma proteins into granules. This makes the platelet a potential novel source of biomarkers for many disease states. Approaches to sample preparation for proteomic studies for biomarkers search vary. Compared with traditional two-dimensional polyacrylamide gel electrophoresis systems, nonelectrophoretic proteomics methods that employ offline protein fractionation methods such as the differential detergent fractionation method have clear advantages. Here we report a proteomic survey of the canine platelet proteome using differential detergent fractionation coupled with mass spectrometry and functional modeling of the canine platelet proteins identified. A total of 5,974 unique proteins were identified from platelets, of which only 298 (5%) had previous experimental evidence of in vivo expression. The use of offline prefractionation of canine proteins by differential detergent fractionation resulted in greater proteome coverage as compared with previous reports. This initial study contributes to a broader understanding of canine platelet bi...Continue Reading

Citations

Jan 30, 2020·Animals : an Open Access Journal From MDPI·Laura CorteseAlessandra Pelagalli
Dec 2, 2020·BMC Veterinary Research·Pinar LeventZeki Yilmaz

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Methods Mentioned

BETA
electrophoresis
reverse phase chromatography

Software Mentioned

PSORTII
! Tandem
Ingenuity Pathway Analysis
DDF2GO
AgBase
EASY
TMbase
TMpred14
GORetriever
ProteoWizard

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