PMID: 9545549Apr 18, 1998Paper

Identification of carbohydrate deficient transferrin forms by MALDI-TOF mass spectrometry and lectin ELISABiochim Biophys Acta 1998 Aug 24;1381(3):356

Biochimica Et Biophysica Acta
J PeterW Hösel

Abstract

Transferrin was isolated from sera of patients with severe alcohol abuse and from control sera by affinity chromatography using an immobilized polyclonal antibody from sheep, followed by gel filtration. The purified transferrin was then separated by MonoQ chromatography. Compared to the controls, sera from heavy alcohol consumers showed two additional transferrin peaks, eluting earlier than the three main transferrin forms present in all sera. Further analysis of the isolated transferrin forms by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and enzyme linked immunosorbent assay with different digoxigenylated lectins (lectin ELISA) revealed that the main carbohydrate deficient transferrin (CDT) forms are lacking either one or both of the N-Glycan chains.

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Citations

Jul 26, 2005·Analytical and Bioanalytical Chemistry·Sandra Arizaga RodríguezAlfredo Sanz-Medel
Aug 25, 2001·Analytical Biochemistry·H R BergenS Naylor
May 16, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anders Helander, Jonas P Bergström
Oct 12, 2016·Scientific Reports·Andreas J HülsmeierThierry Hennet
Jun 7, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anna AldénIngvar Rydén
Sep 8, 2007·Molecular & Cellular Proteomics : MCP·Andreas J HülsmeierThierry Hennet
Jan 8, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Peter KleinertHeinz Troxler
Feb 24, 2001·Alcoholism, Clinical and Experimental Research·P SillanaukeeR Z Litten
Nov 19, 2013·Journal of Cellular Biochemistry·Michael Welti, Andreas J Hülsmeier
Oct 28, 2003·Clinical Chemistry·Anders HelanderJan-Olof Jeppsson
Dec 22, 2016·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Scott H StewartRaymond F Anton
Dec 17, 2010·World Journal of Hepatology·Duygu D Harrison-Findik
Jan 22, 2009·Analytica Chimica Acta·M Estela del Castillo BustoAlfredo Sanz-Medel

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