PMID: 8950323Jan 1, 1996Paper

Identification of carcinoembryonic antigen mRNA in circulating peripheral blood of pancreatic carcinoma and gastric carcinoma patients

Life Sciences
N O FunakiM Imamura

Abstract

To detect adenocarcinoma cells in the circulating peripheral blood, we "analyzed the presence of carcinoembryonic antigen (CEA) mRNA in the peripheral blood obtained from patients with pancreatic carcinoma (PC) or with gastric carcinoma (GC) and also, as controls, from pancreatitis or gastritis patients without carcinomas, a gastric lymphoma patient and four healthy volunteers. Because of the small number of carcinoma cells expected in the peripheral blood, the analysis was performed by the reverse transcription followed by an original two-step polymerase chain reaction. By this sensitive method, 3 of 9 PC patients and 2 of 9 GC patients were positive for CEA mRNA. Except for 1 highly advanced PC patient, 3 of 4 CEA mRNA-positive patients developed recurrence after curative resection or liver metastasis after palliative operation within 9 months after the analysis. None of the control patients was positive for CEA mRNA in the peripheral blood. The results suggest that our sensitive RT-PCR method for detecting CEA mRNA in the peripheral blood is practically useful to find the hematogenous spreading of adenocarcinoma cells.

References

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Citations

Mar 5, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Angelo AquinoEnzo Bonmassar
Aug 26, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R A Ghossein, S Bhattacharya
Feb 27, 2001·Biomolecular Engineering·B BöckmannM Giesing
Apr 23, 2005·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Farid E Ahmed
Jul 19, 2002·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Ilka VogelBernd Kremer
Apr 4, 2014·World Journal of Gastroenterology : WJG·Masahiro TsujiuraEigo Otsuji
Nov 17, 2006·Annals of the New York Academy of Sciences·Eirini PapadopoulouGeorgios Nasioulas
Jul 1, 2016·Computational and Structural Biotechnology Journal·Suthee RapisuwonAnton Wellstein
Jun 20, 2019·Expert Review of Molecular Diagnostics·Ka Wan Emily CheungSze Chuen Cesar Wong
Aug 1, 2015·World Journal of Gastroenterology : WJG·Takahiro KishikawaKazuhiko Koike
Jul 20, 2016·World Journal of Gastroenterology : WJG·Taisuke ImamuraEigo Otsuji
Jul 29, 2006·World Journal of Gastroenterology : WJG·Akimasa NakaoShin Takeda
Aug 8, 2020·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Johan Vad-NielsenAnders Lade Nielsen
Feb 21, 2019·Acta Cytologica·Susana Junqueira-NetoSónia A Melo

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