PMID: 9445372Mar 7, 1998Paper

Identification of cardiac oxidoreductase(s) involved in the metabolism of the lipid peroxidation-derived aldehyde-4-hydroxynonenal

The Biochemical Journal
S SrivastavaA Bhatnagar

Abstract

The aim of this study was to identify the cardiac oxidoreductases involved in the metabolism of 4-hydroxy-2-trans-nonenal (HNE), an alpha,beta unsaturated aldehyde generated during the peroxidation of omega-6 polyunsaturated fatty acids. In homogenates of bovine, human and rat ventricles the primary pyridine coenzyme-linked metabolism of HNE was associated with NADPH oxidation. The NADPH-dependent enzyme catalysing HNE reduction was purified to homogeneity from bovine heart. The purified enzyme displayed kinetic and immunological properties identical with the polyol pathway enzyme aldose reductase (AR), and catalysed the reduction of HNE to its alcohol 1,4-dihydroxynonene (DHN), with a Km of 7+/-2 microM. In the presence of NADP the enzyme did not catalyse the oxidation of DHN. During catalysis, HNE did not cause inactivation of AR. Nevertheless when the apoenzyme was incubated with HNE a dissociable complex was formed between the enzyme and HNE, followed by irreversible loss of activity. Inactivation of the enzyme by HNE was prevented by NADP. Partial modification of the enzyme with HNE led to a 17-fold increase in the KHNEm and Kglyceraldehydem, and the HNE-modified enzyme had a 500-fold higher IC50 for sorbinil than for the ...Continue Reading

Citations

Nov 19, 2010·The Journal of Biological Chemistry·Ganesaratnam K BalendiranDuilio Cascio
Jan 24, 2019·Oxidative Medicine and Cellular Longevity·Leonie G M WijermarsEric R Gross
Jul 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·J RuefA Bhatnagar
Apr 28, 2006·Proteins·Ranvir SinghSatish K Srivastava
Jan 25, 2007·Molecular BioSystems·Daniel ConklinAruni Bhatanagar
Oct 25, 2008·Drug Metabolism Reviews·Oleg A BarskiAruni Bhatnagar
Nov 19, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·J Christopher StatesAaron Barchowsky
Oct 7, 2004·The Journal of Biological Chemistry·Sanjay SrivastavaAruni Bhatnagar
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Sanjay SrivastavaSumanth D Prabhu
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Aug 9, 2016·Frontiers in Endocrinology·Emilie PastelA Marie Lefrançois-Martinez
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