Identification of Carnobacterium spp. and Leuconostoc spp. in meat by genus-specific 16S rRNA probes

Letters in Applied Microbiology
H NissenR Dainty

Abstract

Oligonucleotide probes specific for Carnobacterium and Leuconostoc species were constructed from the variable regions of 16S rRNA obtained from the literature and sequence data bases. The probes were hybridized with crude nucleic acid extract from 32 type strains of lactic acid bacteria (LAB) commonly found on meat. Two of the probes hybridized only to the four Carnobacterium species whereas the other two hybridized only to five of the six Leuconostoc species tested. The probes were also hybridized with nucleic acids from unknown strains of LAB. The identification was consistent with the results of biochemical tests used to characterize the two genera.

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Citations

Sep 23, 2003·Journal of Microbiological Methods·Jichan JangHongui Han
Jun 17, 2000·International Journal of Food Microbiology·L VoldH Nissen
Mar 17, 2001·International Journal of Food Microbiology·M A WasneyD S Jayas
Jan 5, 2002·International Journal of Food Microbiology·F M NattressL P Baker
Mar 1, 2003·Meat Science·Céline Larrouture-ThiveyratMarie-Christine Montel
Aug 31, 2000·Applied and Environmental Microbiology·N ben Omar, F Ampe
Jul 9, 2004·Journal of Applied Microbiology·M C MaciánR Aznar
Jul 13, 2005·FEMS Microbiology Letters·Catherine Cailliez-GrimalJean-Bernard Millière
May 25, 2004·Journal of Economic Entomology·G D Inglis, A C Cohen
Mar 15, 2019·Current Pharmaceutical Biotechnology·Gokila Thangavel, Subramaniyan Thiruvengadam
Sep 12, 2019·Food Science of Animal Resources·Gokila Thangavel, Thiruvengadam Subramaniyam

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