Identification of catalytically active groups of Penicillium canescens F-436 beta-galactosidase

Biochemistry. Biokhimii︠a︡
O S KorneevaI V Cheryomushkina

Abstract

The functional groups of Penicillium canescens F-436 beta-galactosidase have been identified. The pK values and heats of ionization of these groups and photoinactivation of the enzyme with methylene blue indicate that the active site contains carboxyl and imidazole groups. A mechanism for the participation of these groups in the cleavage of the glycoside bond in lactose is proposed.

Citations

Dec 17, 2008·Carbohydrate Research·Barbora BrazdovaVyacheslav V Samoshin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.