PMID: 21790023Jul 28, 2011

Identification of catalytically active groups of pea (Pisum sativum L.) beta-glucosidase

Prikladnaia biokhimiia i mikrobiologiia
A N Ershova, O N Barkalova


Functional groups ofcytoplasmic pea beta-glucosidase pretreated to an electrophoretically homogeneous state were identified. Data on the pH dependence of the enzyme activity, calculated heat of ionization, photoinactivation of the enzyme in the presence of methylene blue, and inactivation of the enzyme with diethyl pyrocarbonate suggest that the catalytic site of beta-glucosidase contains the carboxyl group of glutamic or aspartic acids and the imidazole group of histidine.


Related Concepts

Imidazole sodium
Aspartic Acid, Magnesium-Potassium (2:1:2) Salt
Emulsion beta-D-Glucosidase
Diethyl Pyrocarbonate
Enzyme Activation
Hydrogen-Ion Concentration
Urolene Blue

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