Identification of CD21-binding peptides with phage display and investigation of binding properties of HPMA copolymer-peptide conjugates

Bioconjugate Chemistry
Hui DingJindřich Kopeček

Abstract

Cancer targeting with peptides has become promising with the emergence of combinatorial peptide techniques such as phage display. Using phage display under stringent screening conditions, we selected five distinct peptides that specifically recognized the CD21 receptor, a cell surface marker of malignant B cell lymphoma. Two highly hydrophobic sequences were excluded (RLAYWCFSGLFLLVC and PVAAVSFVPYLVKTY). The binding affinity toward CD21 of the other three selected peptides (RMWPSSTVNLSAGRR, PNLDFSPTCSFRFGC, and GRVPSMFGGHFFFSR) was analyzed with fluorescence quenching. Their dissociation constants were determined to be within the micromolar range. On the basis of the results of phage ELISA, competitive phage ELISA, and fluorescence quenching, the binding sites of the three selected peptides were found to reside within the first four short consensus repeats of CD21 (SCR1-4). The peptide RMWPSSTVNLSAGRR (P1) was bound to the N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer, a potential drug carrier for chemotherapeutic agents, and the surface binding properties of HPMA copolymer-P1 conjugates were investigated. Specific interactions were observed between HPMA copolymer-P1 conjugates and surface-bound receptor. Binding of HPMA ...Continue Reading

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Citations

Feb 23, 2010·Chemical Reviews·Susan L Deutscher
Aug 3, 2007·Journal of Drug Targeting·Hui DingJindrich Kopecek
Apr 1, 2007·Expert Opinion on Drug Discovery·Lauren Rh Krumpe, Toshiyuki Mori
Apr 22, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jiyuan Yang, Jindřich Kopeček
Jan 12, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Twan LammersGert Storm
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Rudolph L JulianoHyun Min Kang
May 24, 2016·Advanced Drug Delivery Reviews·Ruiwu LiuKit S Lam
Jul 21, 2015·Biomaterials Science·Te-Wei Chu, Jindřich Kopeček
Mar 10, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Hong XuChuanbin Mao
Aug 1, 2020·Advanced Drug Delivery Reviews·Jindřich Kopeček, Jiyuan Yang
Sep 21, 2007·Molecular Pharmaceutics·Olulanu H AinaKit S Lam
Sep 24, 2010·Bioconjugate Chemistry·Guangchang ZhouStephen J Kron

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