Identification of CD36 as a new surface marker of marginal zone B cells by transcriptomic analysis

Molecular Immunology
Ping ZhangHua Han

Abstract

Follicular (FO) B cells and marginal zone (MZ) B cells belong to the mature B cell population in spleen of mice. To identify new surface markers of these mature B cell subsets, we compared gene expression profiles of FO and MZ B cells by DNA microarray using FACS-sorted mouse FO and MZ B cells. From 14,000 mouse genes, 27 membrane proteins were expressed mainly in MZ B cells while another 22 membrane proteins expressed largely in FO B cells. Using FACS analysis, we identified that CD36, CD68, and CD49e were expressed on MZ B cells but not on FO B cells. In addition, using semi-quantitative PCR, we found that the mRNA of CD131 were much more abundant in MZ B cells. These results revealed new phenotypic properties of MZ and FO B cells, and would facilitate further studies in the differentiation and functions of these mature B cells.

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Citations

Aug 21, 2013·Immunology Letters·Robert D BerahovichThomas J Schall
Sep 1, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Alexander J A DeutschPeter Neumeister
Feb 22, 2011·Molecular Immunology·Emilie Domange JordöMikael C I Karlsson
Aug 29, 2007·European Journal of Immunology·Amber C Donahue, David A Fruman
Dec 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Woong-Jai WonJohn F Kearney
Feb 9, 2017·Lipids in Health and Disease·Laura C Echeverri Tirado, Lina M Yassin
Jun 12, 2020·International Journal of Molecular Sciences·Harald ManggeGunter Almer

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