Abstract
Follicular (FO) B cells and marginal zone (MZ) B cells belong to the mature B cell population in spleen of mice. To identify new surface markers of these mature B cell subsets, we compared gene expression profiles of FO and MZ B cells by DNA microarray using FACS-sorted mouse FO and MZ B cells. From 14,000 mouse genes, 27 membrane proteins were expressed mainly in MZ B cells while another 22 membrane proteins expressed largely in FO B cells. Using FACS analysis, we identified that CD36, CD68, and CD49e were expressed on MZ B cells but not on FO B cells. In addition, using semi-quantitative PCR, we found that the mRNA of CD131 were much more abundant in MZ B cells. These results revealed new phenotypic properties of MZ and FO B cells, and would facilitate further studies in the differentiation and functions of these mature B cells.
References
Sep 20, 1991·Cell·T KitamuraA Miyajima
Jul 14, 1989·Cell·P OquendoB Seed
Sep 1, 1995·Stem Cells·V Pistoia, A Corcione
Jun 28, 1996·The Journal of Biological Chemistry·M D NavazoJ L McGregor
May 23, 1998·Annual Review of Immunology·W P ArendC Gabay
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·S P ColganR S Blumberg
Jul 27, 2000·The Journal of Biological Chemistry·C T CoburnN A Abumrad
Mar 10, 2001·Blood·W M MiaoJ Lawler
Aug 18, 2001·Science·B SchiemannM L Scott
Aug 24, 2001·Nature·Y FukuiT Sasazuki
Sep 19, 2001·The Journal of Clinical Investigation·M FebbraioR L Silverstein
Nov 7, 2001·The Journal of Experimental Medicine·S C Finnemann, R L Silverstein
Mar 27, 2002·International Reviews of Immunology·J C PoeT F Tedder
Apr 23, 2002·Nature Immunology·Kenji TanigakiTasuku Honjo
Jul 20, 2002·Science·Theresa T Lu, Jason G Cyster
Sep 5, 2002·European Journal of Immunology·Michele E YoudRonald B Corley
May 20, 2003·Immunity·Toshiki SaitoHisamaru Hirai
Feb 14, 2004·Immunological Reviews·Thiago Lopes-Carvalho, John F Kearney
Mar 18, 2005·Annual Review of Immunology·Shiv PillaiStewart T Moran
Apr 14, 2005·Seminars in Immunology·Bhaskar SrivastavaDavid Allman
Citations
Aug 21, 2013·Immunology Letters·Robert D BerahovichThomas J Schall
Sep 1, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Alexander J A DeutschPeter Neumeister
Jul 29, 2011·Blood·Franziska HampelUrsula Zimber-Strobl
Jan 18, 2013·PloS One·Weixia LiLi-xin Wang
Feb 22, 2011·Molecular Immunology·Emilie Domange JordöMikael C I Karlsson
Feb 11, 2016·Cell Biology International·Yaozhen ChenXingbin Hu
Nov 24, 2011·Blood·Hongsheng WangHerbert C Morse
Aug 29, 2007·European Journal of Immunology·Amber C Donahue, David A Fruman
Dec 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Woong-Jai WonJohn F Kearney
Feb 9, 2017·Lipids in Health and Disease·Laura C Echeverri Tirado, Lina M Yassin
Jun 12, 2020·International Journal of Molecular Sciences·Harald ManggeGunter Almer