Identification of CD4+ T cell epitopes on the fusion (F) and attachment (G) proteins of bovine respiratory syncytial virus (BRSV)

Vaccine
M H FoggG Taylor

Abstract

To gain insight into the antigenic structure of the F and G proteins of BRSV, we have mapped CD4+ T cell epitopes on these proteins using synthetic peptides and lymphocytes from vaccinated, naturally infected or experimentally infected calves, in proliferation assays. Bovine CD4+ T cells recognised epitopes that were distributed predominantly within the F1 subunit of the F protein, some of which were adjacent to previously identified B cell epitopes. Bovine CD4+ T cell epitopes within the G protein were mainly located within the cytoplasmic tail. Several immunodominant bovine T cell epitopes within the F protein, that were recognised by calves with different haplotypes, are also recognised by human T cells. Thus, cattle and humans appear to recognise similar T cell epitopes on the F protein. Studies using antibodies to bovine MHC class II and BoLA DR-transfected CHO cells as antigen-presenting cells indicated that immunodominant regions of the F and G proteins contained both DR- and DQ-restricted epitopes. The finding that there was little recognition of the extracellular domain of the G protein by T cells has important implications for vaccine design based on the soluble form of this protein.

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Citations

Jun 30, 2005·Animal Health Research Reviews·Elizabeth J Glass
Apr 16, 2004·Clinical Microbiology Reviews·Andrew J EastonHelene F Rosenberg
Sep 4, 2004·Journal of the American Veterinary Medical Association·Matthew R Sandbulte, James A Roth
Jul 14, 2010·The Veterinary Clinics of North America. Food Animal Practice·Bruce W Brodersen
Jan 18, 2005·Vaccine·Geraldine TaylorLewis H Thomas
May 31, 2011·Veterinary Immunology and Immunopathology·Elizabeth J GlassOliver C Jann
Jan 3, 2015·Molecular Immunology·Efrain Guzman, Geraldine Taylor
Jan 4, 2003·Nature Reviews. Immunology·Wayne R Hein, Philip J Griebel
Sep 19, 2002·The Pediatric Infectious Disease Journal·Martin C J Kneyber, Jan L L Kimpen

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