Identification of cell types in human diseased corneas

Cornea
M C KenneyDonald Brown

Abstract

Activated myofibroblasts and macrophages are often found in corneal wound models. The current study was performed to determine whether human diseased corneas that had active tissue remodeling and enzyme activities also possessed myofibroblasts, macrophages, major histocompatibility complex class II cells, and/or CD-68-positive cells. Normal, keratoconus, keratoconus with hydrops, bullous keratopathy, map-dot-fingerprint dystrophy, failed grafts, and acid burn/neovascularized corneas were collected, frozen in OCT, sectioned, and stained with antibodies to alpha smooth muscle actin (myofibroblast marker), CD14 (macrophage marker), CD68 (lysosomal membrane marker), and HLA-DR (major histocompatibility complex class II cells). Selective histochemical stains identified lysosomal enzymes. Normal and map-dot-fingerprint dystrophy corneas lacked antibody and enzyme staining. Keratoconus corneas were positive for CD68, HLA-DR, and lysosomal enzymes but were negative for CD14 and smooth muscle actin. Bullous keratopathy corneas had CD68-, CD14-, and HLA-DR-positive cells, relatively normal enzyme levels, and were smooth muscle actin-negative. Failed graft corneas had significant numbers of CD68-, CD14-, and HLA-DR-positive cells and incr...Continue Reading

References

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Citations

Nov 24, 2005·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·M Cristina Kenney, Donald J Brown
Nov 30, 2006·Cornea·Raja NarayananM Cristina Kenney
Oct 1, 2013·Investigative Ophthalmology & Visual Science·André A M TorricelliSteven E Wilson
Aug 19, 2015·Acta Ophthalmologica·Jessica H MathewJan P G Bergmanson
Dec 14, 2011·Journal of Cataract and Refractive Surgery·Naoyuki MorishigeKoh-Hei Sonoda
Aug 4, 2015·The Ocular Surface·Robert P L WisseAllegonda Van der Lelij
May 2, 2015·Eye·V GalvisA Acera
Oct 9, 2012·Experimental Eye Research·Lubica DudakovaKaterina Jirsova
Oct 23, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A V LjubimovM C Kenney
Feb 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rajeev BuddiDonald J Brown
Jan 12, 2019·Journal of Refractive Surgery·Rohit ShettyArkasubhra Ghosh
Sep 24, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Cristina KenneyAlexander V Ljubimov

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