PMID: 2510154Oct 1, 1989Paper

Identification of cellular proteins that bind to the human immunodeficiency virus type 1 trans-activation-responsive TAR element RNA

Proceedings of the National Academy of Sciences of the United States of America
A GatignolK T Jeang

Abstract

Human immunodeficiency virus type 1 (HIV-1) trans-activator protein Tat activates the expression of its viral long terminal repeat (LTR) through a target transactivation-responsive element termed TAR. We have constructed cell lines that constitutively express the HIV-1 Tat protein. Analyses of nuclear proteins from these cells and from matched control cells that do not express Tat have identified three proteins that bind to a radiolabeled HIV-1 TAR RNA probe. These polypeptides are 100 kDa, 62 kDa, and 46 kDa in size. Competition experiments using a wild-type TAR RNA sequence, a biologically inactive mutant sequence of TAR, and an unrelated RNA species demonstrated that these proteins show higher binding affinity to wild-type TAR than to the other two non-trans-activatable sequences. We hypothesize that these cellular proteins may mediate a function necessary in Tat-dependent activation of the LTR. The fact that no differences were seen in the binding profiles of nuclear proteins to TAR RNA in Tat-producing and Tat-nonproducing cells suggests that Tat does not directly interact with TAR.

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Citations

Jan 1, 1990·Molecular Biology Reports·I W Mattaj
Jan 1, 1991·Biochimie·J KarnM J Gait
Jan 31, 1991·The New England Journal of Medicine·W C Greene
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D N SenGuptaR H Silverman
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M G CordingleyA J Schlabach
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Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·U DellingN Sonenberg
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Feb 19, 2013·Trends in Genetics : TIG·Alfredo CastelloThomas Preiss
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