PMID: 20657729Jul 27, 2010Paper

Identification of Chemically Sulfated/desulfated Glycosaminoglycans in Contaminated Heparins and Development of a Simple Assay for the Detection of Most Contaminants in Heparin.

Glycobiology Insights
Jing PanLijuan Zhang

Abstract

Contaminated heparin was linked to at least 149 deaths and hundreds of adverse reactions. Published report indicates that heparin contaminants were a natural impurity, dermatan sulfate, and a contaminant, oversulfated chondroitin sulfate (OSCS). OSCS was assumed to derive from animal cartilage. By analyzing 26 contaminated heparin lots from different sources, our data indicate that the heparin contaminants were chemically sulfated or chemically sulfated/desulfated glycosaminoglycans (GAGs) consisting of heparan sulfate, chondroitin sulfate, and dermatan sulfate based on monosaccharide quantification, CE, heparin lyase digestion, and liquid chromatography/mass spectrometry analysis. Since currently recommended heparin quality control assays had failed to detect certain heparin contaminants, a simple method that detects most contaminants in heparin was developed. This assay detects specific heparin structures that most contaminants cannot mimic and can be performed in any laboratory equipped with an UV spectrometer.

Citations

Sep 21, 2010·Analytical Biochemistry·Zhenling LiuRobert J Linhardt
Apr 25, 2012·IEEE Transactions on Pattern Analysis and Machine Intelligence·Omar OreifejMubarak Shah
May 15, 2012·Journal of Pharmaceutical and Biomedical Analysis·Pierre A J MourierChristian Viskov
May 15, 2013·Sensors·Jie-Ci YangJiann-Jone Chen
Jun 16, 2010·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis· Yi Qian Lijuan Zhang
Jun 16, 2010·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Jawed FareedRussell D Hull
Mar 10, 2010·Nature Biotechnology·Jing PanLijuan Zhang

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