PMID: 236Jul 1, 1975

Identification of chloride-binding sites in hemoglobin by nuclear-magnetic-resonance quadrupole-relaxation studies of hemoglobin digests

European Journal of Biochemistry
E ChianconeJ Wyman

Abstract

35Cl minus-nuclear magnetic resonance (NMR) studies indicate that various digests of human hemoglobin with carboxypeptidase A and B, or a combination of the two, may be used for the identification of chloride binding sites. All the digestion products contain, like hemoglobin itself, at least two classes of binding sites, one of high, the others of low affinity. The pH dependence of the excess linewidth of the 35Cl minus NMR signal indicates that in the simple digests with either carboxypeptidase A or B, chloride is bound with high affinity at or near His-beta146-Asp-beta94 and at or near Val-alpha1-Arg-alpha141. The high-affinity sites show, in the case of the simple digests, a strong oxygen linkage which is lost in the forms digested with both carboxypeptidase A and B; this linkage may thus be correlated to the presence of conformational changes. Organic phosphates, like inositol hexaphosphate, show competition for some of the high-affinity chloride binding sites in hemoglobin and in the simple digests. This competition is likewise lost in the doubly digested hemoglobins.

References

Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J BonaventuraJ Wyman
Jan 1, 1972·Cold Spring Harbor Symposia on Quantitative Biology·M F Perutz, L F TenEyck
Jan 1, 1972·Cold Spring Harbor Symposia on Quantitative Biology·J V Kilmartin, J A Hewitt
Feb 5, 1974·Journal of Molecular Biology·J BonaventuraJ WYMAN

Citations

Jul 12, 1978·Biophysics of Structure and Mechanism·M Brumen, S Svetina
Jan 1, 1982·The International Journal of Biochemistry·K Horiuchi
Oct 1, 1979·Respiration Physiology·P GuesnonB Bohn
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E S Hazard, Q H Gibson
Jul 15, 1985·European Journal of Biochemistry·G AmiconiM Brunori
Dec 17, 2009·Analytical Biochemistry·Zhibin MaiXiaoyong Zou
Oct 12, 2004·Biophysical Journal·Ken VictorR G Bryant
May 19, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·P TosquiM F Colombo
Sep 8, 1987·Biochemistry·W J FantlJ M Manning
Jan 8, 1999·Journal of Molecular Biology·J J EnglanderS W Englander
Apr 15, 1980·Journal of Molecular Biology·M F PerutzH S Rollema
Sep 14, 2000·The Journal of Biological Chemistry·C H TaboyA L Crumbliss
Dec 1, 1985·Digestive Diseases and Sciences·A E Rogers, K M Nauss
Jan 29, 1999·Physiological Reviews·D C DawsonM K Mansoura
Jun 16, 2001·Journal of Applied Physiology·H D PrangeB Pinshow

Related Concepts

Carbon Monoxide
Carboxypeptidase
Chloride Ion Level
Eryhem
Hydrogen-Ion Concentration
Mathematics
In Vivo NMR Spectroscopy
Osmolality
Peptide Fragments
Phytin

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