Identification of Chloride Channels CLCN3 and CLCN5 Mediating the Excitatory Cl- Currents Activated by Sphingosine-1-Phosphate in Sensory Neurons

Frontiers in Molecular Neuroscience
Yanmei QiMichaela Kress

Abstract

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in numerous physiological and pathophysiological processes. We have previously reported a S1P-induced nocifensive response in mice by excitation of sensory neurons via activation of an excitatory chloride current. The underlying molecular mechanism for the S1P-induced chloride conductance remains elusive. In the present study, we identified two CLCN voltage-gated chloride channels, CLCN3 and CLCN5, which mediated a S1P-induced excitatory Cl- current in sensory neurons by combining RNA-seq, adenovirus-based gene silencing and whole-cell electrophysiological voltage-clamp recordings. Downregulation of CLCN3 and CLCN5 channels by adenovirus-mediated delivery of shRNA dramatically reduced S1P-induced Cl- current and membrane depolarization in sensory neurons. The mechanism of S1P-induced activation of the chloride current involved Rho GTPase but not Rho-associated protein kinase. Although S1P-induced potentiation of TRPV1-mediated ionic currents also involved Rho-dependent process, the lack of correlation of the S1P-activated Cl- current and the potentiation of TRPV1 by S1P suggests that CLCN3 and CLCN5 are necessary components for S1P-induced excitatory Cl- current...Continue Reading

References

Jan 1, 1989·Journal of Cellular Physiology·S G KremerK L Skorecki
Dec 29, 1995·The Journal of Biological Chemistry·K SteinmeyerT J Jentsch
Jan 5, 1999·The Journal of Biological Chemistry·T FriedrichT J Jentsch
Jul 6, 2000·The Biochemical Journal·S Pyne, N J Pyne
Mar 29, 2001·The Journal of Biological Chemistry·P HuangD J Nelson
Mar 28, 2002·Physiological Reviews·Thomas J JentschAnselm A Zdebik
Jan 28, 2003·Pflügers Archiv : European journal of physiology·Willy GüntherThomas J Jentsch
May 3, 2003·Nature Reviews. Molecular Cell Biology·Sarah Spiegel, Sheldon Milstien
May 13, 2004·The Journal of Biological Chemistry·Mari KonoRichard L Proia
Dec 8, 2004·The European Journal of Neuroscience·Elizabeth K Joseph, Jon D Levine
Feb 16, 2005·Annual Review of Physiology·Thomas J JentschAnselm A Zdebik
May 26, 2005·Current Opinion in Neurobiology·Thomas J JentschOlaf Scheel
Aug 3, 2005·Journal of Lipid Research·Motohiro TaniYasuyuki Igarashi
Nov 30, 2005·Molecular and Cellular Biology·Kiyomi MizugishiRichard L Proia
Sep 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mallorie PoëtThomas J Jentsch
Oct 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Poulami MitraSarah Spiegel
Nov 7, 2006·Prostaglandins & Other Lipid Mediators·Helmut SchmidtGerd Geisslinger
Sep 18, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Daniel GilbertFrank Möhrlen
Apr 4, 2008·Journal of Neurophysiology·Héctor I Rocha-GonzálezFrancisco J Alvarez-Leefmans
Jun 28, 2008·Annals of Clinical Biochemistry·Ryunosuke OhkawaYutaka Yatomi
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wen-Qi LaiBernard P Leung
Feb 27, 2009·Experimental Brain Research·Marshall Devor
May 1, 2009·Molecular Biology of the Cell·Lee StirlingAnthony D Morielli
Sep 25, 2009·Molecular Biology of the Cell·Bas PonsioenWouter H Moolenaar
Jun 4, 2010·Clinical and Investigative Medicine. Médecine Clinique Et Experimentale·Jianfang FuQiuhe Ji
Aug 31, 2010·Cytoskeleton·Mutsuki AmanoKozo Kaibuchi
Feb 4, 2011·American Journal of Physiology. Cell Physiology·Alia ShatanawiR William Caldwell

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Citations

Apr 24, 2020·Frontiers in Neuroscience·Bettina U WilkeMichaela Kress

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Methods Mentioned

BETA
Infection
fluorescence imaging
PCR
electrophoresis
GTPase
imaging technique
GTPases

Software Mentioned

Patchmaster
OriginPro7
HEKA
Graphpad Prism
MetaFluor ®
Fitmaster

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