Identification of chronological and photoageing-associated microRNAs in human skin

Scientific Reports
Ankit SrivastavaAndor Pivarcsi

Abstract

MicroRNAs are short non-coding RNAs that play key roles in regulating biological processes. In this study, we explored effects of chronological and photoageing on the miRNome of human skin. To this end, biopsies were collected from sun-exposed (outer arm, n = 45) and sun-protected (inner arm, n = 45) skin from fair-skinned (phototype II/III) healthy female volunteers of three age groups: young, 18-25 years, middle age, 40-50 years and aged, > 70 years. Strict inclusion criteria were used for photoageing scoring and for chronological ageing. Microarray analysis revealed that chronological ageing had minor effect on the human skin miRNome. In contrast, photoageing had a robust impact on miRNAs, and a set of miRNAs differentially expressed between sun-protected and sun-exposed skin of the young and aged groups was identified. Upregulation of miR-383, miR-145 and miR-34a and downregulation of miR-6879, miR-3648 and miR-663b were confirmed using qRT-PCR in sun-exposed skin compared with sun-protected skin. qRT-PCR analysis revealed that miR-383, miR-34a and miR-134 were differentially expressed in all three age groups both in chronological and photoageing, suggesting a synergetic effect of intrinsic and extrinsic ageing on their exp...Continue Reading

References

Sep 1, 1989·Journal of the American Academy of Dermatology·B A Gilchrest
Sep 19, 2002·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Dominique BatisseJean-Luc Lévêque
May 20, 2003·The British Journal of Dermatology·S BossetJ F Nicolas
Sep 17, 2004·Nature·Victor Ambros
Dec 7, 2007·Genes & Development·Adam S AdlerHoward Y Chang
Feb 8, 2008·Journal of Cosmetic Dermatology·Carl R Thornfeldt
Apr 19, 2008·Clinical and Experimental Dermatology·E SonkolyA Pivarcsi
Mar 19, 2009·Photodermatology, Photoimmunology & Photomedicine·Inês Alencar de CastroLucio Bakos
Sep 18, 2009·The Journal of Investigative Dermatology·Enikö SonkolyAndor Pivarcsi
Sep 25, 2009·The Journal of Investigative Dermatology·Adam GiangrecoFiona M Watt
Jan 22, 2010·Aging Cell·Matthias HacklJohannes Grillari
Jan 22, 2010·The Journal of Investigative Dermatology·Dario AntoniniCaterina Missero
Mar 6, 2010·Experimental Dermatology·Line Marie HolstRobert Gniadecki
May 7, 2010·The Journal of Investigative Dermatology·Maranke I Koster
Aug 12, 2010·Experimental Dermatology·Tianling WeiEnikö Sonkoly
Sep 10, 2010·The Journal of Investigative Dermatology·Janosch HildebrandAnja Knott
Feb 2, 2012·Journal of Cell Science·Thalyana Smith-Vikos, Frank J Slack
Aug 17, 2012·The Journal of Investigative Dermatology·Peter DynoodtMireille J L Van Gele
Sep 18, 2012·Cellular and Molecular Life Sciences : CMLS·Matthew S Ning, Thomas Andl
Jul 10, 2013·Current Drug Targets·Deeba N SyedHasan Mukhtar
Mar 29, 2014·Cell Death & Disease·J KimY Xu
May 3, 2014·Ageing Research Reviews·Mara ManciniEleonora Candi
Feb 17, 2015·The Journal of Investigative Dermatology·Dongqing LiNing Xu Landén
Feb 28, 2015·Genome Biology·Marina LizioUNKNOWN FANTOM consortium
Apr 16, 2015·Molecular Medicine·Christiane GrasConstança Figueiredo
Jul 4, 2015·Aging Cell·Reuben H KimNo-Hee Park
Nov 26, 2015·IUBMB Life·Fatima FahsQing-Sheng Mi
Dec 8, 2015·Journal of Dermatological Science·Marek SvobodaTomáš Muthný
Feb 24, 2016·Journal of Dermatological Science·Tong LiLeihong Xiang
Aug 23, 2017·Nature Biotechnology·Derek de RieMichiel J L de Hoon

❮ Previous
Next ❯

Citations

Jul 30, 2020·International Journal of Molecular Sciences·Marta GerasymchukIgor Kovalchuk
Feb 14, 2021·Environmental Toxicology and Pharmacology·Lucia RackovaZuzana Brnoliakova
Feb 13, 2021·Journal of Personalized Medicine·Loubna MaziniGabriel Malka
Mar 16, 2021·The Journal of Clinical Investigation·Asurayya WorredeAshani T Weeraratna

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
chip

Software Mentioned

Venny
SAM
Prism
Enrichr
MEV ( Multiple Experiment Viewer )
TargetScan

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.