Identification of Circular RNA Expression Profiles and their Implication in Spinal Cord Injury Rats at the Immediate Phase.

Journal of Molecular Neuroscience : MN
Yadong LiuBin Liu

Abstract

This study aimed to explore the implication of circular RNA (circRNA) expression profiles in spinal cord injury (SCI) rats at the immediate phase. CircRNA expression profiles in spinal cord samples from five SCI rats at the immediate phase (2 h post SCI) and five sham control (Ctrl) rats were assessed by microarray analysis. Subsequently, ten candidate circRNAs (obtained from microarray analysis) were validated in ten SCI rats at the immediate phase and ten Ctrl rats by the reverse transcription quantitative polymerase chain reaction (RT-qPCR). PCA plots and heatmap analyses revealed that circRNA expression profiles could distinguish SCI rats at the immediate phase from Ctrl rats. Furthermore, 1101 circRNAs were upregulated and 897 circRNAs were downregulated in SCI rats at the immediate phase compared with Ctrl rats. These dysregulated circRNAs distributed on all chromosomes, and most of them located on chromosome 1-10. As for circRNA types, most of these dysregulated circRNAs were exonic. Additionally, enrichment analyses displayed that these dysregulated circRNAs were enriched in multiple signaling pathways related to neuronal signal transduction, immunity, and inflammation, such as the calcium signaling pathway, JAK-STAT si...Continue Reading

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Citations

Feb 19, 2021·Frontiers in Neuroscience·Pei LiJingjing Jiang
Apr 13, 2021·Frontiers in Neuroscience·Lvwan XuLin-Lin Wang
Jul 31, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fan BieMin Lan
Aug 18, 2021·Journal of Orthopaedic Translation· Peng PengShiqing Feng
Oct 29, 2021·Experimental and Therapeutic Medicine·Xiankuan XieKan Xu

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Methods Mentioned

BETA
electrophoresis
PCR
PCA

Software Mentioned

miRanda
GraphPad
R
limma
GraphPad Prism
pheatmap
Factoextra
Agilent
Volcano Plots

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