Identification of circular RNAs and their alterations involved in developing male Xenopus laevis chronically exposed to atrazine

Chemosphere
Linlin SaiCheng Peng

Abstract

Atrazine (AZ) is an environmental endocrine disrupting chemical which can affect the development of amphibians. In our past studies, we demonstrated that chronical exposure to 100 μg/L AZ can cause abnormalities in development and related genes expression of gonads in developing male Xenopus laevis (X. laevis) tadpoles. Recent studies by others have demonstrated that circular RNAs (circRNAs) are implicated in multiple developmental anomalies. However, whether circRNAs involve in the effects in AZ-exposed X. laevis remains unknown. In this study, over 68575 circRNAs were detected by circRNA sequencing of testis tissues from control groups (n = 3) and AZ-treated X. laevis (n = 3). Treatment of AZ led to 405 circRNAs differentially expressed including 44 upregulated and 361 downregulated compared with froglets in the control groups. Two upregulated and 6 downregulated circRNAs were further validated by real-time PCR assay which displayed consistent regulation patterns as shown by the transcriptome sequencing results. Two hundreds and eighty two differentially expressed circRNAs played miRNA sponges roles. Kyoto Encyclopedia of Genes and Genomes pathway analysis of miRNA targets showed that AZ-affected circRNAs are mainly involved ...Continue Reading

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Citations

Jun 10, 2019·Environmental and Molecular Mutagenesis·Bambarendage P U PereraDana C Dolinoy
Sep 25, 2020·Environmental Science and Pollution Research International·Rodrigo de Souza AntônioAngélica Marquetotti Salcedo Vieira
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Mar 14, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yu ZhangMingyou Li
Jul 9, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Xiao MaXuejun Li

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