Identification of cis and trans-acting transcriptional regulators in chondroinduced fibroblasts from the pre-phenotypic gene expression profile.

Gene
Karen E Yates

Abstract

Cell differentiation is regulated via expression of successive sets of genes. In an in vitro model of chondrocyte differentiation, human dermal fibroblasts (hDFs) cultured in collagen sponges are induced to express cartilage matrix genes after 7 days' culture with demineralized bone powder (DBP). A shift in expression of many other genes occurs within 3 days, before chondroblast phenotypic genes are detectable. In this study, the pre-chondrogenic gene expression profile was used as a starting point to derive information on transcriptional regulation of chondrocyte differentiation induced by DBP. Putative cis regulatory elements were identified by comparing promoter regions from three genes that are highly upregulated in chondroinduced hDFs (BIGH3, COL1A2, and FN1) [Zhou, S., Glowacki, J., Yates, K.E, 2004. Comparison of TGF-beta/BMP pathways signaled by demineralized bone powder and BMP-2 in human dermal fibroblasts. J. Bone Min. Res. 19, 1732-1741] and whose products are known to interact in the matrix [Kim, J.E., et al., 2002. Molecular properties of wild-type and mutant betaIG-H3 proteins. Investig. Ophthalmol. Vis. Sci. 43, 656-661]. The effect of DBP on nuclear protein binding to cis elements was measured with an array-bas...Continue Reading

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Citations

Mar 1, 2012·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Dec 25, 2010·Cardiovascular Research·Jianbin ShenLi Li
Feb 10, 2007·Experimental Cell Research·Sonsoles Piera-VelazquezSergio A Jimenez
Dec 15, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shuanhu ZhouJulie Glowacki
Jun 8, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Taku UkaiJoji Mochida
Jan 27, 2009·The Journal of Craniofacial Surgery·Julie GlowackiShuichi Mizuno

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