Identification of class II ADP-ribosylation factors as cellular factors required for hepatitis C virus replication

Cellular Microbiology
Rayan FarhatYves Rouillé

Abstract

GBF1 is a host factor required for hepatitis C virus (HCV) replication. GBF1 functions as a guanine nucleotide exchange factor for G-proteins of the Arf family, which regulate membrane dynamics in the early secretory pathway and the metabolism of cytoplasmic lipid droplets. Here we established that the Arf-guanine nucleotide exchange factor activity of GBF1 is critical for its function in HCV replication, indicating that it promotes viral replication by activating one or more Arf family members. Arf involvement was confirmed with the use of two dominant negative Arf1 mutants. However, siRNA-mediated depletion of Arf1, Arf3 (class I Arfs), Arf4 or Arf5 (class II Arfs), which potentially interact with GBF1, did not significantly inhibit HCV infection. In contrast, the simultaneous depletion of both Arf4 and Arf5, but not of any other Arf pair, imposed a significant inhibition of HCV infection. Interestingly, the simultaneous depletion of both Arf4 and Arf5 had no impact on the activity of the secretory pathway and induced a compaction of the Golgi and an accumulation of lipid droplets. A similar phenotype of lipid droplet accumulation was also observed when GBF1 was inhibited by brefeldin A. In contrast, the simultaneous depletio...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 7, 2003·Current Biology : CB·Subba Rao Gangi SettyChristopher G Burd
Jan 16, 2004·Gastroenterology·Takanobu KatoTakaji Wakita
Mar 3, 2004·Journal of Virology·Anne Op De BeeckJean Dubuisson
Dec 24, 2004·Molecular Biology of the Cell·Ting-Kuang NiuCatherine L Jackson
Jun 11, 2005·Science·Brett D LindenbachCharles M Rice
Jul 21, 2005·Molecular Biology of the Cell·Laura A Volpicelli-DaleyRichard A Kahn
Oct 17, 2006·PLoS Pathogens·Sara CherryNorbert Perrimon
Apr 6, 2007·Molecular Biology of the Cell·Lee Ann CohenJulie G Donaldson
Jun 14, 2008·PLoS Pathogens·Monique H VerheijeCornelis A M de Haan
Jun 27, 2008·Journal of Virology·Michelle J SnooksDavid A Anderson
Nov 22, 2008·PLoS Pathogens·George A BelovEllie Ehrenfeld
Dec 11, 2008·PLoS Biology·Mathias BellerBrian Oliver
Mar 17, 2009·Cell Host & Microbe·Andrew W TaiRaymond T Chung
Apr 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kristi L BergerGlenn Randall
Apr 22, 2009·Cell Host & Microbe·Sonja WelschRalf Bartenschlager
May 23, 2009·Journal of Cell Science·Krishnakant G SoniJuan S Bonifacino
Jul 18, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maud TrotardJacques Le Seyec
Aug 12, 2009·Molecular Genetics and Genomics : MGG·Quynh Trang BuiCatherine L Jackson
Sep 11, 2009·Journal of Virology·Kjerstin H W LankeFrank J M van Kuppeveld
Nov 13, 2009·Journal of Virology·Lucie GoueslainYves Rouillé
May 21, 2010·The Journal of General Virology·Pauline FerrarisPhilippe Roingeard
May 26, 2010·Cellular Microbiology·George A BelovEllie Ehrenfeld
Nov 12, 2010·Journal of Virology·Meirav MattoBenjamin Aroeti
May 19, 2011·Nature Reviews. Molecular Cell Biology·Julie G Donaldson, Catherine L Jackson
Nov 11, 2011·Journal of Virology·George A BelovEllie Ehrenfeld
Nov 23, 2011·The Journal of Biological Chemistry·Mateusz KudelkoPei Gang Wang
Dec 22, 2011·Cell Structure and Function·Kouhei TakashimaKazuhisa Nakayama
Jun 5, 2012·PloS One·Jianmin WangQi Jin
Aug 15, 2013·Journal of Cell Science·Samuel BouvetCatherine L Jackson

❮ Previous
Next ❯

Citations

Apr 9, 2017·Proceedings of the National Academy of Sciences of the United States of America·Marianne D HansenMarit W Anthonsen
Oct 20, 2017·Journal of Virology·Kirstin VondersteinAnna K Överby
Nov 8, 2017·Cellular Microbiology·Rayan FarhatLaurence Cocquerel
Jul 31, 2018·DNA and Cell Biology·Richard Lindqvist, Anna K Överby
Aug 26, 2017·Virologica Sinica·Na ZhangLeiliang Zhang
Jun 21, 2018·The Journal of General Virology·Juliette FerlinYves Rouillé
Jan 19, 2019·Life Science Alliance·Javier Fernandez-ChamorroEncarnación Martinez-Salas
May 28, 2020·Cells·Noémie OechslinJérôme Gouttenoire
Jun 24, 2018·Viruses·Richard LindqvistAnna K Överby
Feb 24, 2017·Journal of Biosciences·Wulong LiangYanming Zhang
Jun 3, 2021·Viruses·Azman Embarc-BuhEncarnacion Martinez-Salas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.