PMID: 9019735Nov 1, 1996Paper

Identification of ClC-2-like chloride currents in pig pancreatic acinar cells

Pflügers Archiv : European journal of physiology
M A Carew, P Thorn


We used the whole-cell patch-clamp technique to identify a hyperpolarization-activated Cl- current (approximately 50 pA/pF at -60 mV) in acutely isolated, voltage-clamped, single, pig pancreatic acinar cells. This current had characteristic properties of inward rectification, a Cl- = Br->I- selectivity sequence and activation by extracellular hypotonicity. These properties are similar to those reported for the ClC-2 Cl- channel recently cloned from rat and expressed in oocytes. An antiserum raised against the C-terminus of ClC-2 localized the channel to secretory granules containing amylase that were situated exclusively at the apical pole of the pig pancreatic acinar cells, but the channel was not localized in the basolateral membrane. Our study combines a functional assessment and immunohistochemical localization of ClC-2-like channels in a native mammalian cell. The data suggest that the ClC-2-like Cl- channel may function as a Cl- efflux pathway in pancreatic acinar cells.


Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
May 6, 2009·Clinical and Experimental Pharmacology & Physiology·Natasha BehrendorffPeter Thorn
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·E M SchwiebertG R Cutting
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May 17, 2002·The Journal of Physiology·Jorge ArreolaJames E Melvin
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Jun 30, 2005·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Marie L KellyBhanu P Jena
May 31, 2018·Physiological Reviews·Thomas J Jentsch, Michael Pusch
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Jun 5, 1998·The American Journal of Physiology·S HuberM Horster
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