Identification of cleavage sites and substrate proteins for two mitochondrial intermediate peptidases in Arabidopsis thaliana

Journal of Experimental Botany
Chris CarrieJürgen Soll

Abstract

Most mitochondrial proteins contain an N-terminal targeting signal that is removed by specific proteases following import. In plant mitochondria, only mitochondrial processing peptidase (MPP) has been characterized to date. Therefore, we sought to determine the substrates and cleavage sites of the Arabidopsis thaliana homologues to the yeast Icp55 and Oct1 proteins, using the newly developed ChaFRADIC method for N-terminal protein sequencing. We identified 88 and seven putative substrates for Arabidopsis ICP55 and OCT1, respectively. It was determined that the Arabidopsis ICP55 contains an almost identical cleavage site to that of Icp55 from yeast. However, it can also remove a far greater range of amino acids. The OCT1 substrates from Arabidopsis displayed no consensus cleavage motif, and do not contain the classical -10R motif identified in other eukaryotes. Arabidopsis OCT1 can also cleave presequences independently, without the prior cleavage of MPP. It was concluded that while both OCT1 and ICP55 were probably acquired early on in the evolution of mitochondria, their substrate profiles and cleavage sites have either remained very similar or diverged completely.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1989·European Journal of Biochemistry·G von HeijneR G Herrmann
Apr 1, 1995·Plant Physiology·J WhelanD A Day
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M C WilceJ A Wilce
Dec 11, 1999·Journal of Bioenergetics and Biomembranes·E Glaser, P Dessi
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Aug 23, 2002·Biochimica Et Biophysica Acta·Oleksandr GakhGrazia Isaya
Jul 3, 2003·Biochimica Et Biophysica Acta·Judy HirstJohn E Walker
Feb 14, 2004·Molecular Biology and Evolution·Hwan Su YoonDebashish Bhattacharya
Mar 19, 2004·Current Biology : CB·Torsten KleffmannSacha Baginsky
Oct 7, 2004·Journal of Molecular Biology·Charlotta RudheElzbieta Glaser
Jan 12, 2005·Trends in Plant Science·A Harvey MillarIan M Møller
Oct 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anne-Marie DuchêneIan D Small
Apr 21, 2007·Nature Protocols·Olof EmanuelssonHenrik Nielsen
Apr 17, 2009·The New Phytologist·Jan MitschkeStefan A Rensing
Aug 26, 2009·Cell·Agnieszka ChacinskaNikolaus Pfanner
Sep 2, 2009·The Journal of Biological Chemistry·Adi NaamatiOphry Pines
Sep 8, 2009·Journal of Molecular Biology·Anna-Karin BerglundElzbieta Glaser
Oct 31, 2009·Nature Methods·Niklaas ColaertKris Gevaert
Jun 3, 2010·Journal of Proteome Research·Tine E ThingholmMartin R Larsen
Jul 16, 2010·Trends in Plant Science·Emmanuelle Graciet, Frank Wellmer
Apr 29, 2011·Molecular Biology of the Cell·F-Nora VögtleChris Meisinger
Jun 3, 2011·Protein Science : a Publication of the Protein Society·Alexander Varshavsky
Nov 17, 2011·Physiologia Plantarum·Malgorzata KwasniakHanna Janska
Dec 17, 2011·Biochimica Et Biophysica Acta·Dirk MossmannF-Nora Vögtle
Dec 20, 2011·Trends in Biochemical Sciences·Thomas BeckerNikolaus Pfanner
Apr 13, 2012·Biochimica Et Biophysica Acta·Pedro Filipe Teixeira, Elzbieta Glaser

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Citations

Oct 18, 2015·Molecular Biology and Evolution·Jan ManiAndré Schneider
Jan 17, 2016·Biochimica Et Biophysica Acta·Alan WaltonKris Gevaert
May 11, 2016·Trends in Plant Science·Chun Pong Lee, A Harvey Millar
Apr 22, 2016·Physiologia Plantarum·Beata KmiecElzbieta Glaser
Sep 7, 2016·Cell and Tissue Research·Daniel Poveda-HuertesF Nora Vögtle
May 12, 2017·The New Phytologist·Fatih DemirPitter Florian Huesgen
May 2, 2018·Proteomics·Sonali R BhagwatAmit Kumar
Sep 10, 2016·Journal of Cell Science·Chris CarrieJürgen Soll
May 30, 2020·Molecular & Cellular Proteomics : MCP·Eduard HofsetzPitter F Huesgen
Jul 19, 2018·The Biochemical Journal·Abi S GhifariMonika W Murcha
Mar 2, 2019·Annual Review of Plant Biology·Etienne H MeyerChris Carrie
Sep 1, 2018·Doklady. Biochemistry and Biophysics·A S BaikE S Pojidaeva
Nov 19, 2019·Journal of Experimental Botany·Abi S GhifariMonika W Murcha
Sep 2, 2020·Proceedings of the National Academy of Sciences of the United States of America·Robert S AllenCraig C Wood
Jan 17, 2021·FEBS Letters·Maria Gomez-Fabra Gala, Friederike-Nora Vögtle
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Eduard HofsetzPitter F Huesgen
May 11, 2020·Mitochondrion·Shaobai HuangA Harvey Millar
Aug 28, 2020·Plant Physiology and Biochemistry : PPB·Salvador González-GordoFrancisco J Corpas

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Methods Mentioned

BETA
in vitro transcription
PCR
acetylation
confocal microscopy

Software Mentioned

Mascot
ProtParam
Proteome Discoverer
SequenceLogo
Protein Calculator
BLAST
ChaFRADIC
Icelogo
TargetP
SUBAcon

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