Identification of Clinically Relevant Mycobacterial Species After Extended Incubation Times in the BACTEC MGIT System

American Journal of Clinical Pathology
Bryan ReaKevin Alby

Abstract

Traditionally, for mycobacterial culture both solid and broth media are used and routinely held for 6 weeks minimum to optimize yield. We retrospectively reviewed all positive mycobacterial cultures over a 12-month period to assess growth kinetics of clinically relevant isolates. From January to December 2015, 658 positive mycobacteria cultures by solid (7H11 and 7H10 plates) and/or broth (BACTEC MGIT) media were identified and reviewed. In broth-only cultures, 21 of 153 (13.7%) from 21 patients were positive after 28 days' incubation. Subsequent chart review revealed the following species: 11 Mycobacterium avium intracellulare complex (MAI), five Mycobacterium tuberculosis (MTB), and five other non-MTB/MAI mycobacteria. Two of the cases of MTB were first-time isolates, and 11.4% of MTB-positive cultures became positive after 4 weeks' incubation. These data provide strong evidence reaffirming that clinically meaningful results are frequently detected after extended incubation times by broth-only methods, including several MTB isolates.

References

Jun 30, 2000·American Journal of Clinical Pathology·S E SharpR J Poppiti
Nov 23, 2007·The New England Journal of Medicine·Michael C IannuzziAlvin S Teirstein
Oct 12, 2012·Journal of Clinical Microbiology·Gaby E Pfyffer, Franziska Wittwer
Apr 25, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F RageadeA van Belkum
Feb 13, 2016·Journal of Clinical Microbiology·Patricia J SimnerNancy L Wengenack

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