Identification of common differentially-expressed miRNAs in ovarian cancer cells and their exosomes compared with normal ovarian surface epithelial cell cells

Oncology Letters
Shitao ZhangYiling Yang

Abstract

The aim of the present study was to identify common microRNAs (miRNAs) in ovarian cancer (OC) cells and their exosomes using microarray data (accession number GSE76449) available from the Gene Expression Omnibus database, including exosomal samples from 3 OC cell lines, 1 normal ovarian surface epithelial cell line and their original cell samples. Differentially-expressed miRNAs (DE-miRNAs) were identified using the Linear Models for Microarray data method, and mRNA targets of DE-miRNAs were predicted using the miRWalk2 database. The potential functions of the target genes of the DE-miRNAs were analyzed using the Database for Annotation, Visualization and Integrated Discovery tool. The association between crucial miRNAs and target genes, and their clinical associations, were validated using The Cancer Genome Atlas data. As a result, 12 upregulated and 12 downregulated DE-miRNAs were shared by the 3 OC cell lines compared with normal controls in the exosomal samples, while 5 upregulated and 65 downregulated DE-miRNAs were shared between the original cells. Among them, 9 downregulated DE-miRNAs were shared between exosomal and original cells. The target genes of 4 common DE-miRNAs between exosomal and original cells (miR-127-3p, ...Continue Reading

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Citations

Jul 10, 2019·Current Drug Targets·Seyed Mostafa ParizadehAmir Avan
Oct 17, 2019·Journal of Clinical Medicine·Miriam Teeuwssen, Riccardo Fodde
Sep 5, 2020·OncoTargets and Therapy·Jiang-Tao PuTian-Yang Dai
Nov 12, 2020·Current Drug Metabolism·Yin-Xue WangYi-Qing Wang

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Datasets Mentioned

BETA
GSE76449
GPL19117

Methods Mentioned

BETA
RNAseq
nuclear translocation

Software Mentioned

Cytoscape
Limma R - package
LinkedOmics
miRNAMap
edgeR
PITA
RNA22
mirbridge
Database for Annotation , Visualization and Integrated Discove...
GraphPad

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