Identification of Core Gene Expression Signature and Key Pathways in Colorectal Cancer

Frontiers in Genetics
Xiang DingHesheng Luo

Abstract

Colorectal cancer (CRC) is considered the most prevalent malignant tumor that contributes to high cancer-related mortality. However, the signaling pathways involved in CRC and CRC-driven genes are largely unknown. We sought to discover a novel biomarker in CRC. All clinical CRC samples (n = 20) were from Renmin Hospital of Wuhan University. We first selected MAD2L1 by integrated bioinformatics analysis of a GSE dataset. Next, the expression of MAD2L1 in tissues and cell lines was verified by quantitative real-time PCR. The effects of MAD2L1 on cell growth, proliferation, the cell cycle, and apoptosis were examined by in vitro assays. We identified 683 shared DEGs (420 upregulated and 263 downregulated), and the top twenty genes (CDK1, CCNA2, TOP2A, PLK1, MAD2L1, AURKA, BUB1B, UBE2C, TPX2, RRM2, KIF11, NCAPG, MELK, NUSAP1, MCM4, RFC4, PTTG1, CHEK1, CEP55, DTL) were selected by integrated analysis. These hub genes were significantly overexpressed in CRC samples and were positively correlated. Our data revealed that the expression of MAD2L1 in CRC tissues is higher than that in normal tissues. MAD2L1 knockdown significantly suppressed CRC cell growth by impairing cell cycle progression and inducing cell apoptosis. MAD2L1, as a nov...Continue Reading

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Citations

Nov 13, 2020·OncoTargets and Therapy·Xinxin ZhangJianguang Jia
May 15, 2021·Nature Reviews. Genetics·Alexander J SilverMichael R Savona
May 28, 2021·DNA and Cell Biology·Qian YangWeiguo Dong
Jun 3, 2021·International Journal of Environmental Research and Public Health·Abhijeet R PatilSourav Roy
Jun 3, 2021·International Journal of Environmental Research and Public Health·Urbashi BasnetSourav Roy

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Datasets Mentioned

BETA
GSE117606
GPL25373

Methods Mentioned

BETA
PCR
transfection
chip
flow cytometry

Software Mentioned

Gene Set Enrichment Analysis ( GSEA )
ImageJ
KEGG
gplots
Bioconductor
GSEA
limma R
DAVID
GraphPad Prism
R

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