Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.)

The Plant Pathology Journal
Abhijit Daspute, B Fakrudin

Abstract

Pigeonpea Sterility Mosaic Disease (PSMD) is an important foliar disease caused by Pigeonpea sterility mosaic virus (PPSMV) which is transmitted by eriophyid mites (Aceria cajani Channabasavanna). In present study, a F2 mapping population comprising 325 individuals was developed by crossing PSMD susceptible genotype (Gullyal white) and PSMD resistant genotype (BSMR 736). We identified a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7414) and a repulsion phase marker (IABTPPN7983) co-segregating with PSMD reaction. Screening of 325 F2 population using IABTPPN7 revealed that the repulsion phase marker, IABTPPN7983, was co-segregating with the PSMD responsive SV1 at a distance of 23.9 cM for Bidar PPSMV isolate. On the other hand, the coupling phase marker IABTPPN7414 did not show any linkage with PSMD resistance. Additionally, single marker analysis both IABTPPN7983 (P<0.0001) and IABTPPN 7414 (P<0.0001) re...Continue Reading

References

Apr 17, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C M AvilaA M Torres
Nov 8, 2011·Nature Biotechnology·Rajeev K VarshneyScott A Jackson
Apr 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E JohnsonJ C Martinez-Cruzado
Oct 8, 2014·The Plant Pathology Journal·Abhijit DasputeB M Khadi

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Methods Mentioned

BETA
PCR
BSMR
SMA

Software Mentioned

BSA
MAPMAKER

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