Identification of Cryptosporidium species and genotypes in Scottish raw and drinking waters during a one-year monitoring period.

Applied and Environmental Microbiology
Rosely A B NicholsHuw V Smith

Abstract

We analyzed 1,042 Cryptosporidium oocyst-positive slides (456 from raw waters and 586 from drinking waters) of which 55.7% contained 1 or 2 oocysts, to determine species/genotypes present in Scottish waters. Two nested PCR-restriction fragment length polymorphism (RFLP) assays targeting different loci (1 and 2) of the hypervariable region of the 18S rRNA gene were used for species identification, and 62.4% of samples were amplified with at least one of the PCR assays. More samples (577 slides; 48.7% from raw water and 51.3% from drinking water) were amplified at locus 1 than at locus 2 (419 slides; 50.1% from raw water and 49.9% from drinking water). PCR at loci 1 and 2 amplified 45.4% and 31.7% of samples containing 1 or 2 oocysts, respectively. We detected both human-infectious and non-human-infectious species/genotype oocysts in Scottish raw and drinking waters. Cryptosporidium andersoni, Cryptosporidium parvum, and the Cryptosporidium cervine genotype (now Cryptosporidium ubiquitum) were most commonly detected in both raw and drinking waters, with C. ubiquitum being most common in drinking waters (12.5%) followed by C. parvum (4.2%) and C. andersoni (4.0%). Numerous samples (16.6% total; 18.9% from drinking water) contained...Continue Reading

References

Nov 1, 1995·Applied and Environmental Microbiology·D W JohnsonJ B Rose
Aug 15, 1996·Nucleic Acids Research·P TaberletJ Bouvet
Jul 31, 1999·Applied and Environmental Microbiology·L XiaoA A Lal
Dec 13, 2000·International Journal for Parasitology·R FayerS J Upton
Dec 13, 2000·International Journal for Parasitology·T R SlifkoJ B Rose
Feb 1, 2003·Veterinary Parasitology·Alexandre J da SilvaMark L Eberhard
Jan 1, 1990·Parasitology Today·H V Smith, J B Rose
Jul 28, 2005·Trends in Parasitology·Simone M CacciòHuw V Smith
Aug 9, 2005·Applied and Environmental Microbiology·Jianlin JiangLihua Xiao
Dec 8, 2005·Applied and Environmental Microbiology·Norma J RueckerNorman F Neumann
Aug 4, 2006·Applied and Environmental Microbiology·Rosely A B NicholsHuw V Smith
Oct 17, 2006·Parasitology Today·H V Smith, J B Rose
May 8, 2007·Applied and Environmental Microbiology·Norma J RueckerNorman F Neumann
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Sep 9, 2008·Applied and Environmental Microbiology·Wenli YangLihua Xiao
Jun 9, 2009·Experimental Parasitology·Huw V Smith, Rosely A B Nichols
Jun 16, 2009·Emerging Infectious Diseases·Martin KvácOleg Ditrich
Aug 8, 2009·Veterinary Parasitology·Judit Plutzer, Panagiotis Karanis
Jun 12, 2010·Veterinary Parasitology·Ronald FayerDumitru Macarisin

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Citations

Aug 22, 2012·Parasitology·Una Ryan, Michelle Power
Jan 29, 2013·Applied and Environmental Microbiology·L ConnellyC L Alexander
Jul 6, 2012·Clinical Microbiology Reviews·Stephanie M FletcherJohn Ellis
Jan 23, 2014·Emerging Infectious Diseases·Na LiYaoyu Feng
Mar 13, 2013·Parasite : Journal De La Société Française De Parasitologie·Isaia SotiriadouPanagiotis Karanis
Oct 24, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Norma J RueckerNorman F Neumann
Aug 16, 2011·Trends in Parasitology·Nobuko YoshidaMartin S Llewellyn
Sep 15, 2012·Comparative Immunology, Microbiology and Infectious Diseases·Nada Abu SamraPeter N Thompson
Jul 8, 2011·Preventive Veterinary Medicine·Joshua P A SweenyC Jacobson
Feb 11, 2015·Experimental Parasitology·Marzieh Ezzaty MirhashemiTheo De Waal
Oct 4, 2016·Veterinary Parasitology·Nawal HijjawiUna Ryan
Sep 1, 2015·Journal of Water and Health·Guy RobinsonGraciela I Ramírez Toro
Feb 4, 2016·Journal of Water and Health·Wendy KrkosekGraham A Gagnon
Nov 6, 2020·Environmental Science and Pollution Research International·Hasti DaraeiAmin Mousavi Khaneghah
Jun 22, 2021·Journal of Water and Health·Laurice Beatrice Raphaelle O Dela PeñaWindell L Rivera

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