Identification of cyanidin glycosides as constituents of freeze-dried black raspberries which inhibit anti-benzo[a]pyrene-7,8-diol-9,10-epoxide induced NFkappaB and AP-1 activity

Carcinogenesis
Stephen S HechtSteven G Carmella

Abstract

Dietary freeze-dried black raspberries inhibit tumor induction by N-nitrosomethylbenzylamine in the rat esophagus, but the constituents responsible for this chemopreventive activity have not been identified. We fractionated freeze-dried black raspberries and used mouse epidermal JB6 Cl 41 cells stably transfected with either a nuclear factor kappa B (NFkappaB)- or an activator protein 1 (AP-1)-luciferase reporter, and treated with racemic anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), to assess the inhibitory effects of the fractions. The ethanol and water extracts of the freeze-dried black raspberries had inhibitory activity and these extracts were fractionated by HPLC to give several bioactive fractions. Further HPLC analysis yielded multiple subfractions, some of which inhibited BPDE-induced NFkappaB activity. Major constituents of the most active subfractions were identified by their spectral properties and in comparison with standards as cyanidin-3-O-glucoside, cyanidin 3-O-(2(G)-xylosylrutinoside) and cyanidin 3-O-rutinoside. Analysis of freeze-dried black raspberries indicated that these three components comprised approximately 3.4% of the material by dry weight. Consistent with these results, standard cyanidin-3-O-gl...Continue Reading

Associated Clinical Trials

Aug 31, 2010·Susan Mallery DDS, PhD, Susan Mallery DDS, PhD

References

May 1, 1980·Journal of Agricultural and Food Chemistry·R E WrolstadC F Madero
Mar 22, 2000·Journal of Agricultural and Food Chemistry·H M Merken, G R Beecher
Nov 8, 2001·Carcinogenesis·G D Stoner, A Gupta
Jan 14, 2003·Trends in Molecular Medicine·Matthew R YoungNancy H Colburn
Jan 7, 2004·Journal of Agricultural and Food Chemistry·Tony K McGhieDwayne J Jensen
Mar 12, 2004·Cancer Letters·Florian R Greten, Michael Karin
Apr 7, 2004·The Journal of Cell Biology·Jingxia LiChuanshu Huang
Sep 30, 2004·International Journal of Cancer. Journal International Du Cancer·Farrukh AfaqHasan Mukhtar
Aug 9, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Darren CookeTim Marczylo
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal

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Citations

May 22, 2010·The British Journal of Nutrition·Elena AzziniGiuseppe Maiani
Jun 12, 2008·Carcinogenesis·Gary David StonerBruce Cordell Casto
Jan 14, 2009·Cancer Prevention Research·Li-Shu WangGary D Stoner
Dec 25, 2015·Nutrition and Cancer·Marta Kula, Mirosława Krauze-Baranowska
Jul 15, 2016·International Journal of Molecular Sciences·María Teresa ArizaBruno Mezzetti
Dec 20, 2007·Journal of Cellular Biochemistry·Silvia D StanShivendra V Singh
Nov 13, 2018·Current Medicinal Chemistry·Antonio SpecialeMariateresa Cristani
Aug 28, 2020·Frontiers in Immunology·Samanta C FunesAlexis M Kalergis
Apr 22, 2008·The Journal of Nutrition·Colleen CarkeetJanet A Novotny
Jan 18, 2017·Chemical Research in Toxicology·Karam El-BayoumyJoseph B Guttenplan
Feb 23, 2008·Journal of Agricultural and Food Chemistry·Artemio Z TulioA Raymond Miller

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