PMID: 2495766May 1, 1989Paper

Identification of cytochrome P450a (P450IIA1) as the principal testosterone 7 alpha-hydroxylase in rat liver microsomes and its regulation by thyroid hormones

Archives of Biochemistry and Biophysics
M P Arlotto, A Parkinson

Abstract

In the preceding paper, evidence was presented that rat liver microsomes contain two structurally related isozymes of cytochrome P450, namely cytochromes P450a and P450m, that can both catalyze the 7 alpha-hydroxylation of testosterone. The aim of the present study was to determine the extent to which these two P450 isozymes are responsible for the 7 alpha-hydroxylation of testosterone catalyzed by rat liver microsomes. Four monoclonal antibodies against cytochrome P450a, designated A2, A4, A5, and A7, were prepared in BALB/c mice. Monoclonal antibodies A2 (an IgM), A4 (an IgG2b), and A5 (an IgG1) were determined to be distinct immunoglobulins, whereas A7 could not be distinguished from A5. All of the antibodies were highly specific for cytochrome P450a; none cross-reacted with cytochrome P450m or with 10 other P450 isozymes purified from rat liver microsomes. Competition experiments between unlabeled and horseradish peroxidase-conjugated antibodies revealed that each of the monoclonal antibodies recognized the same epitope on cytochrome P450a. None of the monoclonal antibodies bound to denatured cytochrome P450a, suggesting that they each bound to a spatial epitope. A monospecific, polyclonal antibody against cytochrome P450a ...Continue Reading

References

Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C M SilvaJ A Cidlowski
May 15, 1988·Archives of Biochemistry and Biophysics·J R Halpert
Jul 1, 1987·The Biochemical Journal·K A HostetlerP S Guzelian
May 15, 1987·Archives of Biochemistry and Biophysics·A J SonderfanA Parkinson
Feb 11, 1987·Journal of Immunological Methods·M M McKinney, A Parkinson
Feb 1, 1987·DNA·D W NebertW Levin
Nov 1, 1985·Archives of Biochemistry and Biophysics·L M ReikP E Thomas
Feb 16, 1972·Biochemical and Biophysical Research Communications·A Y Lu, W Levin
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T J SmithA Kappas
Jan 1, 1980·Journal of Immunological Methods·S F de StGroth, D Scheidegger
Aug 1, 1980·European Journal of Biochemistry·F L HochL Ernster

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Citations

May 24, 1991·Biochimica Et Biophysica Acta·S Imaoka, Y Funae
Oct 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·F UshioM Kimura
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P BullockA Parkinson
Oct 6, 1997·Journal of Toxicology and Environmental Health·G H Gunasena, M F Kanz
Oct 2, 2003·Biological & Pharmaceutical Bulletin·Makiko ShimizuMasamichi Fukuoka
Jun 9, 1992·Biochemical Pharmacology·S IkemotoY Funae
Feb 15, 1990·Archives of Biochemistry and Biophysics·M HalvorsonA Parkinson
May 14, 2008·Neurochemical Research·Graziano PinnaErminio Costa
Dec 1, 1991·Archives of Biochemistry and Biophysics·D C Eberhart, A Parkinson

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