Identification of cytoskeletal [14C]carboplatin-binding proteins reveals reduced expression and disorganization of actin and filamin in cisplatin-resistant cell lines

Molecular Pharmacology
Ding-Wu ShenMichael M Gottesman

Abstract

Cisplatin resistant (CP-r) cells often show decreased uptake of cisplatin in association with reduced cell surface proteins and decreased endocytosis. In this report, two major [14C]carboplatin-binding proteins were identified as filamin and actin by photoaffinity labeling and mass spectrometry. Decreased expression of these two proteins was found in two different human CP-r cell lines (KB-CP20 and 7404-CP20), in comparison with their parental cell lines (KB-3-1 and BEL-7404), respectively. Disorganization of beta-actin and filamin 250 and 90 was also detected in these CP-r cells by confocal microscopy. Transfection of a wild-type actin-enhanced green fluorescent protein (EGFP) expression vector into 7404-CP20 cells resulted in a nonfilamentous actin-EGFP distribution compared with a normal distribution in the cisplatin-sensitive BEL-7404 cells, suggesting that cytoskeletal organization is disturbed in the CP-r cells. The identification of actin and filamin as [14C]carboplatin-binding proteins and decreased expression and disorganization of several cytoskeletal proteins in CP-r cells provide a molecular and cellular basis for the known defect in endocytosis in these cells.

Citations

Apr 23, 2009·Pharmaceutical Research·Eliisa MannermaaArto Urtti
Apr 12, 2008·Purinergic Signalling·Christopher A Gabel
Sep 30, 2008·The Journal of Physical Chemistry. B·Christian G CanlasPei Tang
Mar 2, 2010·Chemical Research in Toxicology·Jessica M Weems, Garold S Yost
Jul 9, 2010·Nature·Jonathan A HollanderPaul J Kenny
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xing-Jie LiangLee Jia
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xuebo HuMoonsoo M Jin
Dec 29, 2009·The Journal of Biological Chemistry·Dmitri SamovskiJacob Bar-Tana
Mar 1, 2012·Nucleic Acids Research·Anaïs WanetPatricia Renard
Nov 26, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Wei YueKim L R Brouwer
Feb 22, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Althea ElliottJohn J Reiners
Feb 10, 2009·The Journal of Pharmacology and Experimental Therapeutics·Ryan M PelisNicholas A Delamere
Aug 19, 2010·The Journal of Pharmacology and Experimental Therapeutics·Gary H PerdewTimothy V Beischlag
Oct 1, 2010·American Journal of Physiology. Cell Physiology·Fang LiWenhui Hu
Oct 2, 2009·American Journal of Physiology. Cell Physiology·William G SinkinsWilliam P Schilling
Feb 21, 2006·Cancer Research·Xing-Jie LiangMichael M Gottesman
Aug 30, 2008·Molecular Cancer Research : MCR·Xing-Jie LiangMichael M Gottesman
Jul 23, 2011·Genes & Cancer·David MatallanasWalter Kolch
Dec 21, 2006·Reproductive Biology and Endocrinology : RB&E·Norbert WaltherRichard Ivell
Feb 18, 2012·Journal of Lipid Research·Bella KalderonJacob Bar-Tana
Sep 27, 2013·PLoS Computational Biology·Yun M Zhao, Anthony R French
Jan 6, 2006·Drug Metabolism Reviews·Xianbin TianKim L R Brouwer
Feb 2, 2013·Drug Metabolism Reviews·Eva RamboerMathieu Vinken
Jan 20, 2010·Drug Metabolism Reviews·Katalin JemnitzPeter Krajcsi
Jan 1, 2008·Toxicology Mechanisms and Methods·Peter Ward
Mar 20, 2016·Drug Metabolism and Pharmacokinetics·Ryosuke TakahashiKatsuhiro Kanda
Feb 23, 2011·Biopharmaceutics & Drug Disposition·Han-Joo MaengK Sandy Pang
Aug 1, 2006·Biochemical and Biophysical Research Communications·Verena MaternaHermann Lage

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.