Identification of cytotoxic T lymphocyte epitopes in dengue virus serotype 1

Journal of Medical Virology
Zhi-Liang DuanJin-Sheng Wen

Abstract

Dengue virus (DENV) has a serious and growing impact on global health and the exact role of DENV-specific CD8(+) T-cells in DENV infection is still uncertain. In the present study, SYFPEITHI algorithm was used to screen the amino acid sequence of Dengue virus serotype 1 (DENV-1) for potential epitopes, and seven putative HLA-A*1101-restricted and five putative HLA-A*2402-restricted epitopes conserved in hundreds of DENV-1 strains were synthesized. The binding affinity of these epitope candidates to corresponding HLA molecules was evaluated using competitive peptide-binding assay. The immunogenicity and specificity of peptides were further tested in HLA-A*1101 transgenic mice, HLA-A*2402 transgenic mice and peripheral blood mononuclear cells (PBMCs) of patients infected with DENV-1. Percentage inhibition (PI) values calculated in competitive peptide-binding assay showed that six peptides (E39-47 PTLDIELLK, NS5(505-513) GVEGEGLHK, NS2b(15-23) SILLSSLLK, NS5(561-569) ALLATSIFK, NS3(99-107) AVEPGKNPK, and NS4b(159-167) VVYDAKFEK) could bind to HLA-A*1101 molecule with high affinity and five peptides (NS3472-480 QYIYMGQPL, NS4a40-48 AYRHAMEEL, NS5(880-888) DYMTSMKRF, NS3(548-556) SYKVASEGF, and NS3(22-30) IYRILQRGL) have a high affi...Continue Reading

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Citations

Sep 30, 2017·Cells·Josué da Costa Lima-JuniorFátima Conceição-Silva
Nov 3, 2017·Interdisciplinary Sciences, Computational Life Sciences·Dibyabhaba PradhanArun Kumar Jain
Sep 26, 2019·Frontiers in Immunology·Yuan TianDaniela Weiskopf
Apr 8, 2020·Frontiers in Cellular and Infection Microbiology·Kaihao FengJing An
Apr 26, 2018·Frontiers in Immunology·Sudheer GuptaDebasis Biswas

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