Identification of de novo synthesized and relatively older proteins: accelerated oxidative damage to de novo synthesized apolipoprotein A-1 in type 1 diabetes.

Diabetes
Abdul JaleelK Sreekumaran Nair

Abstract

The accumulation of old and damaged proteins likely contributes to complications of diabetes, but currently no methodology is available to measure the relative age of a specific protein alongside assessment of posttranslational modifications (PTM). To accomplish our goal of studying the impact of insulin deficiency and hyperglycemia in type 1 diabetes upon accumulation of old damaged isoforms of plasma apolipoprotein A-1 (ApoA-1), we sought to develop a novel methodology, which is reported here and can also be applied to other specific proteins. To label newly synthesized proteins, [ring-(13)C(6)]phenylalanine was intravenously infused for 8 h in type 1 diabetic participants (n = 7) during both insulin treatment and 8 h of insulin deprivation and in nondiabetic participants (n = 7). ApoA-1 isoforms were purified by two-dimensional gel electrophoresis (2DGE) and assessment of protein identity, PTM, and [ring-(13)C(6)]phenylalanine isotopic enrichment (IE) was performed by tandem mass spectrometry. Five isoforms of plasma ApoA-1 were identified by 2DGE including ApoA-1 precursor (pro-ApoA-1) that contained the relatively highest IE, whereas the older forms contained higher degrees of damage (carbonylation, deamidation) and far le...Continue Reading

References

Sep 1, 1991·The Journal of Clinical Investigation·P De FeoM W Haymond
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G GhiselliB C Sherrill
Oct 31, 1983·Biochemical and Biophysical Research Communications·G GhiselliB C Sherrill
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·E KimS G Young
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·L J YanR S Sohal
Jul 29, 1998·Free Radical Biology & Medicine·I GrattaglianoE Altomare
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·L J Yan, R S Sohal
Jun 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J OrpiszewskiM D Benson
Dec 7, 2000·Diabetes Research and Clinical Practice·A TelciA Sivas
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A ChavousC M O'Connor
Oct 19, 2004·Journal of the American College of Cardiology·Petra J Mateos-CáceresLuis A Rico
Sep 4, 2008·American Journal of Physiology. Endocrinology and Metabolism·Abdul JaleelK Sreekumaran Nair
May 26, 2009·Biochimica Et Biophysica Acta·K Krishna Sharma, Puttur Santhoshkumar
Aug 6, 2009·American Journal of Physiology. Endocrinology and Metabolism·Abdul JaleelK Sreekumaran Nair

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Citations

May 29, 2012·Journal of Cardiovascular Translational Research·Linda R PetersonJean E Schaffer
Mar 14, 2013·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Rozalina G McCoy, K Sreekumaran Nair
Jul 13, 2012·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·M L Johnson, K S Nair
Apr 2, 2014·Oxidative Medicine and Cellular Longevity·Daniel Ortuño-SahagúnArgelia E Rojas-Mayorquín
Apr 9, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Judit CubedoLina Badimon
Nov 17, 2011·Ageing Research Reviews·Brian A IrvingK Sreekumaran Nair
Feb 8, 2011·Biochimica Et Biophysica Acta·M H Vendelbo, K S Nair
Dec 17, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Gemma VilahurLina Badimon
Apr 3, 2015·Analytical and Bioanalytical Chemistry·Kelly M HinesK Sreekumaran Nair
Mar 4, 2015·Metabolism: Clinical and Experimental·Brian A IrvingK Sreekumaran Nair
Sep 30, 2010·Diabetes·Daniel S Ory, Jean E Schaffer
Aug 11, 2016·Metabolism: Clinical and Experimental·Shankarappa ManjunathaK Sreekumaran Nair
Oct 28, 2017·The Journal of Clinical Endocrinology and Metabolism·Sangeeta R KashyapTakhar Kasumov
Jun 6, 2018·American Journal of Physiology. Endocrinology and Metabolism·Antoinette MoranK Sreekumaran Nair
Jan 17, 2019·Cardiovascular & Hematological Disorders Drug Targets·Y DurgaraoM S Rukmini
Aug 23, 2017·Molecular and Cellular Biochemistry·Rai Ajit K Srivastava
Jul 3, 2019·Frontiers in Nutrition·Joseph W BealsStephan van Vliet
Jul 13, 2017·American Journal of Physiology. Endocrinology and Metabolism·Sean A NewsomMatthew M Robinson
Apr 15, 2011·The New England Journal of Medicine·Anindya Gupta
May 1, 2021·Cells·Blake J CochranKerry-Anne Rye
Jul 6, 2021·Journal of Diabetes and Metabolic Disorders·Liliane de Paula SilvaDayane Alberton

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Methods Mentioned

BETA
electrophoresis
environmental stresses
deamidation

Software Mentioned

Mascot
BioWorks

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