PMID: 3750846Oct 15, 1986Paper

Identification of defects in the neuraminidase gene of four temperature-sensitive mutants of A/WSN/33 influenza virus

Virology
T J Bos, D P Nayak

Abstract

Four influenza (A/WSN/33) mutants, temperature sensitive (ts) for neuraminidase (NA) (Sugiura et al., 1972, 1975) were analyzed. All four ts mutants were found to be defective at the nonpermissive temperature (39.5 degrees) both in enzymatic activity and in transport to the cell surface. Upon shift down to the permissive temperature (33 degrees), enzymatic activity and transport to the cell surface were both restored suggesting that the mutational defect is reversible. Comparative sequence analysis of the NA gene from ts mutants, their revertants and wild type WSN viruses revealed that in each case single point mutations causing amino acid substitutions were associated with the ts defect. The positions of each point mutation when mapped in the three-dimensional structure of NA varied. However, all four amino acid substitutions were located in beta-sheet strands of the head region. Several other amino acid changes not essential for the ts phenotype were found in each mutant NA. The nonessential changes were localized either in the stalk region or in the loop structures of the head, but none in the beta-sheet strands. Because both enzymatic activity and transport of NA were affected in all four mutants, we propose that the mutati...Continue Reading

References

Mar 1, 1976·Journal of Virology·P Palese, J L Schulman
Jan 1, 1985·Current Topics in Microbiology and Immunology·P M Colman, C W Ward
Feb 1, 1968·Journal of Virology·E D KilbourneR G Webster
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A R DavisD P Nayak
Aug 25, 1982·Nucleic Acids Research·D R Bentley, G G Brownlee
Mar 1, 1957·Biochimica Et Biophysica Acta·A GOTTSCHALK

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Citations

Jan 1, 1994·Microbiology and Immunology·F Yamamoto-GoshimaK Maeno
Nov 8, 2014·Journal of Virology·Diogo V da SilvaRobert Daniels
Feb 1, 1994·Journal of Virology·F Yamamoto-GoshimaS Yoshii
Dec 1, 1986·Journal of Virology·A K PattnaikD P Nayak

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