Identification of defects in the transcriptional program during lineage-specific in vitro differentiation of CD34(+) cells selected from patients with both low- and high-risk myelodysplastic syndrome

Experimental Hematology
Saskia GuellerWolf-K Hofmann

Abstract

Development of myelodysplastic syndrome (MDS) is suggested to follow a multistep pathogenesis and is characterized by accumulation of molecular defects of the hematopoietic stem/progenitor cells, resulting in aberrant differentiation and proliferation. To detect alterations within the transcriptional program in MDS-derived CD34(+) cells during lineage-specific differentiation, we performed serial gene expression analysis of in vitro differentiated erythro-, granulo-, and megakaryopoietic cells using oligonucleotide microarrays (HG-U133A, Affymetrix, Santa Clara, CA, USA). For selected genes, expression data were confirmed using real-time polymerase chain reaction. We identified genes with altered expression during lineage-specific differentiation in either low- or high-risk MDS cells compared to the expression patterns of continuously up- or downregulated genes from the normal transcriptional program of hematopoiesis. In cluster analyses, we could show that MDS samples have a distinct expression pattern of a set of selected genes compared to normal cells, which allows prediction of the affiliation of a sample to one group. Furthermore, this study gives an overview of genes that are differentially expressed in MDS cells compared...Continue Reading

References

May 26, 1999·The New England Journal of Medicine·M L Heaney, D W Golde
Oct 3, 1999·The Journal of Biological Chemistry·M A FrevelA E Reeve
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·H NakagawaA de La Chapelle
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Lawrence J Marnett, Raymond N DuBois
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 15, 2002·International Journal of Hematology·Wolf K Hofmann, H Phillip Koeffler
Apr 3, 2003·International Journal of Cancer. Journal International Du Cancer·Vera A E Van LimptRogier Versteeg
Apr 8, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Basem M AbdallahMoustapha Kassem
May 19, 2004·British Journal of Haematology·Andrea PellagattiJacqueline Boultwood
Jan 22, 2005·Annual Review of Medicine·Wolf-K Hofmann, H Phillip Koeffler
Jan 19, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Claudia D BaldusGerhard Ehninger
Mar 15, 2006·International Journal of Hematology·Shinji NakaoMasao Tomonaga

❮ Previous
Next ❯

Citations

Nov 20, 2013·AIDS Research and Therapy·Gero HütterHarald Klüter
Aug 8, 2014·Cellular and Molecular Life Sciences : CMLS·Leonidas BenetatosEleftheria Hatzimichael
Oct 15, 2014·Hematological Oncology·Howard Lopes RibeiroRonald Feitosa Pinheiro
Dec 16, 2010·Leukemia Research·Gero HütterWolf-Karsten Hofmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis