Identification of defense-related rice genes by suppression subtractive hybridization and differential screening

Molecular Plant-microbe Interactions : MPMI
L XiongY Yang

Abstract

Identification of host genes involved in defense responses is one of most critical steps leading to the elucidation of disease resistance mechanisms in plants. In this study, two different cloning strategies were employed to identify defense-related genes from a tropical japonica rice cultivar (Oryza sativa cv. Drew). With the use of bacterial colony arrays, differential screening of a blast fungus (Pyricularia grisea)-induced rice cDNA library led to the isolation of 22 distinct rice genes that are expressed differentially in response to blast infection. Sequence analysis indicates that most of them are full-length cDNAs encoding pathogenesis-related proteins or other relatively abundant proteins. In combination with treatments of cycloheximide plus jasmonic acid (JA) or benzothiadiazole (BTH) in rice seedlings, the polymerase chain reaction-based suppression subtractive hybridization also was conducted to search for immediate early (IE) defense-related genes whose transcription is independent of de novo protein synthesis. The initial screening of only 768 subtracted clones resulted in the identification of 34 distinct IE genes that are induced by JA, BTH, and/or blast infection. Database searches revealed that these IE genes ...Continue Reading

References

Apr 1, 1991·Molecular & General Genetics : MGG·Q Zhu, C J Lamb
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Jul 1, 1997·Genes & Development·Y YangD F Klessig
Sep 2, 1998·Molecular Plant-microbe Interactions : MPMI·D M HorvathN H Chua
Mar 6, 1999·Current Opinion in Plant Biology·P J Rushton, I E Somssich
Mar 6, 1999·Current Opinion in Plant Biology·X Dong
Jun 8, 1999·Chemistry and Physics of Lipids·W J van Blitterswijk, B Houssa
Aug 25, 1999·Current Opinion in Plant Biology·P PiffanelliP Schulze-Lefert
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KawasakiK Shimamoto
Oct 26, 1999·Journal of Molecular Biology·A Klug
Oct 26, 1999·Results and Problems in Cell Differentiation·H Hirt
Jan 7, 2000·Molecular Plant-microbe Interactions : MPMI·C HeG L Wang
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·E A van der BiezenJ D Jones
Apr 8, 2000·Molecular Plant-microbe Interactions : MPMI·C Y KimM J Cho
Jul 25, 2000·Biochemical and Biophysical Research Communications·G K AgrawalR Rakwal
Apr 20, 2001·Molecular Plant-microbe Interactions : MPMI·M W LeeY Yang

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Citations

Dec 14, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chang-Jiang ZhaoGuo-Dong Lu
Dec 18, 2004·Plant Molecular Biology·Etienne PauxJacqueline Grima-Pettenati
Nov 8, 2011·Journal of Experimental Botany·Santosh Kumar GuptaTilak Raj Sharma
May 6, 2006·Molecular Plant-microbe Interactions : MPMI·Nathan S Reyna, Yinong Yang
May 13, 2005·Plant Physiology·Chatchawan JantasuriyaratGuo-Liang Wang
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kosaku TakahashiKozo Ochi
Mar 16, 2006·Applied Microbiology and Biotechnology·C Barsalobres-CavallariM Silva-Filho
May 31, 2015·Ecotoxicology and Environmental Safety·Zahra MirzahossiniMajid Sharifi-Tehrani
Feb 20, 2007·Biochemical and Biophysical Research Communications·Kameswara R KottapalliRandeep Rakwal
Dec 17, 2008·The New Phytologist·Ming-Yan CheungHon-Ming Lam
May 1, 2008·Plant Biotechnology Journal·Sonia CampoBlanca San Segundo
Jun 8, 2010·The Plant Journal : for Cell and Molecular Biology·Mitsuko Kishi-KaboshiHirohiko Hirochika
Jan 8, 2013·Molecular Plant·Rita SharmaPamela C Ronald
Aug 21, 2007·Microbiological Research·Vijai BhadauriaYou-Liang Peng
Nov 8, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G LuG-L Wang
Aug 22, 2017·Frontiers in Plant Science·Rakesh KumarAnirudh Kumar
Jun 1, 2017·Functional Plant Biology : FPB·Xiaorong XiaoYinhua Chen

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