Identification of developmentally regulated mesodermal-specific transcript in mouse embryonic metanephros

American Journal of Physiology. Renal Physiology
Yashpal S KanwarE I Wallner

Abstract

Mesodermal-specific cDNA or transcript (MEST) was identified by suppression subtractive hybridization-PCR of cDNA isolated from embryonic day 13 vs. newborn mice kidneys. At day 13 of mouse gestation, a high expression of MEST, with a single approximately 2.7-kb transcript that was exclusively localized to the metanephric mesenchyme was observed. The MEST mRNA expression gradually decreased during the later stages and then abruptly decreased in the newborn kidneys and subsequent postnatal life, after which a very mild expression persisted in the glomerular mesangium. Regression in mRNA expression during embryonic renal development appears to be related to methylation of the MEST gene. Treatment of metanephroi, harvested at day 13 of gestation with MEST-specific antisense oligodeoxynucleotide resulted in a dose-dependent decrease in the size of the explants and the nephron population. This was associated with a selective decrease in MEST mRNA expression and accelerated apoptosis of the mesenchyme. These findings suggest that MEST, a gene with a putative mesenchymal cell-derived protein, conceivably plays a role in mammalian metanephric development.

References

Oct 1, 1992·Genes & Development·Y SagaS Aizawa
Dec 1, 1992·The Journal of Cell Biology·C KosekiQ al-Awqati
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S W LeeR Sager
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G GillilandH F Bunn
Mar 25, 1986·Nucleic Acids Research·K TokunagaS Sakiyama
Dec 8, 1995·Science·D P Barlow
Jul 1, 1995·The Journal of Cell Biology·M DurbeejP Ekblom
Jul 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Bronner-Fraser
Dec 1, 1996·The Journal of Clinical Investigation·Y S KanwarE I Wallner
May 1, 1997·Human Molecular Genetics·S KobayashiF Ishino
Sep 15, 1998·Kidney International. Supplement·D R AbrahamsonT O Daniel
Nov 14, 1998·The American Journal of Physiology·Y S KanwarD R Peterson
Mar 30, 1999·Experimental Cell Research·R L Jirtle
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y S KanwarE I Wallner
Dec 22, 1999·The American Journal of Physiology·Y S KanwarE I Wallner
Feb 19, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·W MayerR Fundele
Jun 24, 2000·European Journal of Biochemistry·F WilkinC Mamelin
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q YangY S Kanwar
Oct 29, 2000·American Journal of Physiology. Renal Physiology·M PohlS K Nigam
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·R O StuartS K Nigam
May 9, 2001·Current Opinion in Nephrology and Hypertension·J Barasch

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Citations

Nov 28, 2008·Endocrinology·Gabriela P FinkielstainJeffrey Baron
May 6, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Yashpal S KanwarFarhad R Danesh
Dec 10, 2009·In Vitro Cellular & Developmental Biology. Animal·Lisa ShawSusan J Kimber
Apr 5, 2003·Kidney International·Yashpal S KanwarJon W Lomasney
Dec 19, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Renata MeszarosPeter Ekblom
Aug 29, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Keith AlvaresArthur Veis
Dec 17, 2004·Journal of Cellular and Molecular Medicine·F AnglaniA D'Angelo
Jun 20, 2003·Current Opinion in Nephrology and Hypertension·George JaradJeffrey R Schelling

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