Identification of different alkane hydroxylase systems in Rhodococcus ruber strain SP2B, an hexane-degrading actinomycete

Journal of Applied Microbiology
A AmouricL Casalot

Abstract

To investigate the alkane-hydroxylating system of isolate SP2B, closely related to Rhodococcus ruber DSM 43338(T) and uncharacterized so far for its alkane degradation genes. Although isolate SP2B and reference strain can grow on by-products from hexane degradation, the type strain R. ruber was unable, unlike SP2B isolate, to use short-chain alkanes, as assessed by gas chromatography. Using PCR with specific or degenerated primers, inverse PCR and Southern blot, two alkane hydroxylase encoding genes (alkB) were detected in both bacteria, which is in agreement with their alkane range. The first AlkB was related to Rhodococcus AlkB7 enzymes and contains a nonbulky residue at a specific position, suggesting it might be involved in medium- and long-chain alkane oxidation. The second partial alkB gene potentially belongs to alkB5-type, which was found in bacteria unable to use hexane. Moreover, a partial P450 cytochrome alkane hydroxylase, thought to be responsible for the hexane degradation, was detected only in the isolated strain. Rhodococcus ruber SP2B should prove to be a promising candidate for bioremediation studies of contaminated sites because of its large degradation range of alkanes. This is the first thorough study on R....Continue Reading

References

Jan 1, 1990·Biodegradation·R J Watkinson, P Morgan
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1994·Critical Reviews in Biotechnology·A M Warhurst, C A Fewson
Nov 11, 1993·Nucleic Acids Research·H Philippe
Mar 9, 1999·Antonie van Leeuwenhoek·M J LarkinI Nagy
Apr 1, 1999·Biotechnology and Bioengineering·M E AcunaS Revah
Feb 24, 2001·Environmental Microbiology·T H SmitsJ B van Beilen
Aug 21, 2001·Biochemical and Biophysical Research Communications·T MaierU Hahn
Mar 2, 2002·Journal of Bacteriology·Theo H M SmitsJan B van Beilen
Jul 12, 2002·Applied Microbiology and Biotechnology·J C PhilpV Wray
Oct 4, 2002·Environmental Technology·W Terán PérezP Christen
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Jan 1, 1963·Biochimica Et Biophysica Acta·J N BAPTISTM J COON
Oct 27, 2004·Bioscience, Biotechnology, and Biochemistry·Tadashi FujiiJunichi Kato
Nov 30, 2005·Journal of Applied Microbiology·S Heiss-BlanquetF Monot
Dec 27, 2005·Bioscience, Biotechnology, and Biochemistry·Mitsutoshi KubotaNorihiko Misawa
Jan 5, 2006·Applied and Environmental Microbiology·Erika L Johnson, Michael R Hyman
Mar 8, 2006·Applied Microbiology and Biotechnology·Mimmi Throne-HolstSergey B Zotchev
May 19, 2006·Journal of Bacteriology·Julia S SabirovaPeter N Golyshin
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael P McLeodLindsay D Eltis
Jan 12, 2007·Applied Microbiology and Biotechnology·Jan B van Beilen, Enrico G Funhoff
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lu FengLei Wang
May 8, 2007·Applied Microbiology and Biotechnology·M VeselýM Pátek
May 17, 2007·Environmental Microbiology·Yong-Hak KimSang-Jong Kim
Aug 4, 2007·Applied Microbiology and Biotechnology·Alexander WentzelMimmi Throne-Holst
Sep 18, 2007·Applied and Environmental Microbiology·Jonathan O SharpLisa Alvarez-Cohen
Feb 22, 2008·Chemosphere·Eun-Hee Lee, Kyung-Suk Cho
Sep 23, 2008·Journal of Bioscience and Bioengineering·Yuka SameshimaHisao Ohtake

❮ Previous
Next ❯

Citations

Dec 3, 2013·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Luciano ProcópioLucy Seldin
Dec 4, 2014·Systematic and Applied Microbiology·András TáncsicsBalázs Kriszt
May 16, 2014·Scientific Reports·Yong NieXiao-Lei Wu
Jul 5, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Daniel Derrossi MeyerFátima Menezes Bento
Jun 27, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rūta StanislauskienėRolandas Meškys
Dec 17, 2018·Applied Microbiology and Biotechnology·Jessica ZampolliPatrizia Di Gennaro
Nov 25, 2020·BMC Microbiology·Sergey E PeltekNikolay A Kolchanov
Feb 24, 2021·World Journal of Microbiology & Biotechnology·Vijaya RaniRajeev Kaushik
Jun 30, 2019·The Science of the Total Environment·Mengjun ZhangYi Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.