PMID: 7009157Jan 1, 1981Paper

Identification of different forms of the murein-bound lipoprotein found in isolated outer membranes of Escherichia coli

European Journal of Biochemistry
J Wensink, B Witholt

Abstract

The identification of the free and murein-bound forms of the Escherichia coli lipoprotein on dodecylsulphate-polyacrylamide gels was systematically investigated by analyzing the low-molecular-weight proteins (Mr less than 20 000) of both cytoplasmic and outer membranes. The free form of the lipoprotein was identified on 15% polyacrylamide gels as the fastest migrating component (Mr = 7200-7500) of isolated outer membranes; it could be separated from a small cytoplasmic membrane protein (Mr = 6500) which was probably identical to the dicyclohexylcarbodiimide binding proteolipid of the membrane-bound ATPase. Lysozyme treatment of both outer membranes and murein sacculi failed to convert the murein-bound lipoprotein into a fragment of uniform size; instead the bound form appeared as a series of bands consisting of lipoprotein bound to one, two,...eight murein subunits. The composition of this ladder depended on the method used to isolate outer membranes. Beside these lipoprotein bands the outer membrane contained two other proteins, III and V; the relation of these proteins to previously described proteins is discussed.

References

Nov 15, 1977·Biochimica Et Biophysica Acta·L De Leij, B Witholt
Feb 1, 1979·European Journal of Biochemistry·E T Palva
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jun 1, 1970·European Journal of Biochemistry·V Braun, H Wolff

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Citations

Oct 1, 1986·Plant Physiology·G H Vos-ScheperkeuterB Witholt
May 15, 1981·European Journal of Biochemistry·J Wensink, B Witholt
Feb 13, 2009·Molecular Microbiology·Mikhail KudryashevFriedrich Frischknecht
May 24, 2008·Geobiology·Y GorbyT J Beveridge
Jun 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A Z HenriksenO G Brakstad
Nov 1, 2016·Archives of Microbiology·Yoshihiro OjimaMasahito Taya
Jun 5, 1984·Journal of Molecular Biology·G H Vos-Scheperkeuter, B Witholt
Jun 1, 1987·Acta Odontologica Scandinavica·E N VasstrandH B Jensen
Mar 1, 1982·European Journal of Biochemistry·J WensinkB Witholt
Jun 1, 1981·European Journal of Biochemistry·J Wensink, B Witholt
Oct 1, 1989·Journal of Bacteriology·H J MarvinB Witholt
Apr 1, 1983·Journal of Bacteriology·J McEwenP M Silverman
Sep 1, 1982·Journal of Bacteriology·N KatsuiI Shibasaki

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