Identification of differential gene expression in Brassica rapa nectaries through expressed sequence tag analysis.

PloS One
Marshall HamptonClay Carter

Abstract

Nectaries are the floral organs responsible for the synthesis and secretion of nectar. Despite their central roles in pollination biology, very little is understood about the molecular mechanisms underlying nectar production. This project was undertaken to identify genes potentially involved in mediating nectary form and function in Brassica rapa. Four cDNA libraries were created using RNA isolated from the median and lateral nectaries of B. rapa flowers, with one normalized and one non-normalized library being generated from each tissue. Approximately 3,000 clones from each library were randomly sequenced from the 5' end to generate a total of 11,101 high quality expressed sequence tags (ESTs). Sequence assembly of all ESTs together allowed the identification of 1,453 contigs and 4,403 singleton sequences, with the Basic Localized Alignment Search Tool (BLAST) being used to identify 4,138 presumptive orthologs to Arabidopsis thaliana genes. Several genes differentially expressed between median and lateral nectaries were initially identified based upon the number of BLAST hits represented by independent ESTs, and later confirmed via reverse transcription polymerase chain reaction (RT PCR). RT PCR was also used to verify the exp...Continue Reading

References

Aug 1, 1990·The Plant Cell·D R SmythE M Meyerowitz
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1969·Anesthesiology·B Brandstater
Dec 25, 2002·Bioinformatics·Chad Creighton, Samir Hanash
Dec 21, 2005·Die Naturwissenschaften·Clay CarterRobert Thornburg
Oct 29, 2009·Journal of Experimental Botany·Jeffrey M RuhlmannClay J Carter
Dec 25, 2009·Sexual Plant Reproduction·Brian W Kram, Clay J Carter

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Citations

Jun 15, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Reyazul Rouf MirRajeev K Varshney
Jul 16, 2013·BMC Plant Biology·Pil Joon SeoSang-Gyu Kim
Feb 25, 2011·Trends in Plant Science·Martin Heil
Apr 5, 2013·The Plant Journal : for Cell and Molecular Biology·Ricci L BenderClay J Carter
May 17, 2019·Functional & Integrative Genomics·Mukesh ChoudharyRajeev K Varshney
Aug 24, 2018·Frontiers in Plant Science·Anthony J SchmittClay J Carter
Nov 29, 2020·Trends in Plant Science·Filip SlavkovićAbdelhafid Bendahmane
Jul 19, 2017·Plant Science : an International Journal of Experimental Plant Biology·Rahul RoyClay J Carter
Feb 16, 2021·Plant Physiology·Elizabeth C ChattBasil J Nikolau
May 29, 2021·The Plant Journal : for Cell and Molecular Biology·Anzu MinamiClay J Carter

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Methods Mentioned

BETA
dissection
cDNA library construction
PCR
electrophoresis

Software Mentioned

Lasergene SeqMan Pro©
blastx
Creative Genomics
ProAssembler
MMN
Expressionist
Basic Local Alignment Search Tool ( BLAST )
BLAST
GeneData
Affymetrix

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