PMID: 16514277Mar 4, 2006Paper

Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage

The Korean Journal of Parasitology
Kyu-Lee HanTai-Soon Yong

Abstract

Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.

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Citations

Aug 5, 2009·Experimental Parasitology·Ying-Hua XuanHyun-Hee Kong
Oct 28, 2016·The Journal of Eukaryotic Microbiology·Bibiana Chávez-MunguíaAdolfo Martínez-Palomo

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