Identification of differentially expressed genes induced by hydroxyurea in reticulocytes from sickle cell anaemia patients

Clinical and Experimental Pharmacology & Physiology
L S MoreiraF F Costa

Abstract

1. The major effect associated with hydroxyurea (HU) treatment of sickle cell anaemia (SCA) patients is an increase in fetal haemoglobin (HbF) synthesis, which inhibits the polymerization of haemoglobin S. 2. Hydroxyurea improves clinical symptoms by reducing the frequency of pain and vaso-occlusive crises, acute chest syndrome, transfusion requirements and hospitalization. 3. The molecular mechanisms responsible for HU-mediated induction of fetal globin transcription are not completely understood. Therefore, the aim of the present study was to identify differentially expressed genes participating in these mechanisms. 4. We established two suppression subtractive hybridization (SSH) libraries from reticulocytes obtained from SCA patients either not on or on HU treatment. The gene expression of some of the genes identified was subsequently evaluated by real-time polymerase chain reaction (PCR). 5. Genes identified with altered expression included SUDS3, FZD5 and PHC3, which may be associated with the regulation of globin expression. 6. This is the first demonstration of an association between HU treatment and the expression of genes identified in erythroid cells.

References

Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Sep 11, 1997·The New England Journal of Medicine·H F Bunn
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·T IkutaM D Cappellini
Mar 23, 2002·Genome Génome / Conseil National De Recherches Canada·Norma PaniegoH Esteban Hopp
Aug 9, 2002·Molecular and Cellular Biology·Stuart S LevineRobert E Kingston
Jan 25, 2003·British Journal of Haematology·Christina Halsey, Irene A Roberts
Apr 4, 2003·JAMA : the Journal of the American Medical Association·Martin H SteinbergMichael Terrin
May 2, 2003·Molecular and Cellular Biology·Tracey C FleischerDonald E Ayer
Dec 19, 2003·Nucleic Acids Research·Todd W HarrisLincoln D Stein
May 25, 2005·Experimental Hematology·Scott C CrableKathleen P Anderson
Apr 12, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Betty S Pace, Sima Zein

❮ Previous
Next ❯

Citations

Apr 4, 2012·Clinical and Experimental Pharmacology & Physiology·Mehdi BananHossein Najmabadi
Mar 5, 2014·Hematology/oncology Clinics of North America·Frans A Kuypers
Dec 15, 2010·Expert Review of Proteomics·Susan YuditskayaGregory J Kato
Mar 3, 2021·Orphanet Journal of Rare Diseases·Nirmani YasaraSachith Mettananda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.