Identification of Differentially Expressed Genes in Different Types of Broiler Skeletal Muscle Fibers Using the RNA-seq Technique.

BioMed Research International
Han WangAyong Zhao

Abstract

The difference in muscle fiber types is very important to the muscle development and meat quality of broilers. At present, the molecular regulation mechanisms of skeletal muscle fiber-type transformation in broilers are still unclear. In this study, differentially expressed genes between breast and leg muscles in broilers were analyzed using RNA-seq. A total of 767 DEGs were identified. Compared with leg muscle, there were 429 upregulated genes and 338 downregulated genes in breast muscle. Gene Ontology (GO) enrichment indicated that these DEGs were mainly involved in cellular processes, single organism processes, cells, and cellular components, as well as binding and catalytic activity. KEGG analysis shows that a total of 230 DEGs were mapped to 126 KEGG pathways and significantly enriched in the four pathways of glycolysis/gluconeogenesis, starch and sucrose metabolism, insulin signalling pathways, and the biosynthesis of amino acids. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to verify the differential expression of 7 selected DEGs, and the results were consistent with RNA-seq data. In addition, the expression profile of MyHC isoforms in chicken skeletal muscle cells showed that...Continue Reading

References

Nov 1, 1990·Pflügers Archiv : European journal of physiology·D Danieli-BettoM Midrio
Jul 1, 1996·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·F SchwägeleK O Honikel
Apr 12, 2000·Acta Physiologica Scandinavica·G Van Hall
Jun 22, 2000·Molecular Cell Biology Research Communications : MCBRC·P T LoughnaC Brownson
Sep 22, 2000·Microscopy Research and Technique·E Bandman, B W Rosser
Dec 26, 2002·Biology of the Cell·Shuichi MachidaRumiko Matsuoka
Mar 1, 1962·The American Journal of Anatomy·J M STEIN, H A PADYKULA
Nov 19, 2004·American Journal of Physiology. Cell Physiology·Claudio F PerezPaul D Allen
Dec 17, 2005·Biochemical and Biophysical Research Communications·H SanchezA Peinnequin
Jun 8, 2006·Annual Review of Biochemistry·Rhonda Bassel-Duby, Eric N Olson
Feb 2, 2008·Science in China. Series C, Life Sciences·HongMei HuYing Wu
Dec 17, 2008·The Journal of Cell Biology·Belinda S CowlingChristina A Mitchell
Feb 6, 2010·Genome Biology·Matthew D YoungAlicia Oshlack
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Sep 1, 2011·Diabetes Research and Clinical Practice·George DimitriadisSotirios A Raptis
Aug 26, 2011·Intervirology·Gianni CapalboUrban J Scheuring
Oct 21, 2011·Journal of Muscle Research and Cell Motility·David M BrownJohn M Brameld
Nov 19, 2011·Cold Spring Harbor Symposia on Quantitative Biology·Y XiongK-L Guan
Feb 1, 2009·Animal : an International Journal of Animal Bioscience·C JurieJ F Hocquette
Nov 28, 2012·Cell Communication & Adhesion·Chunyu BaiWeijun Guan
Sep 7, 2013·PloS One·Diane Angonin, Terence J Van Raay
Mar 13, 2014·Genetics and Molecular Research : GMR·J T ShuH F Li
Aug 31, 2014·Developmental Biology·Ruth AmannBeat Trueb
Feb 19, 2015·Nature Biotechnology·Mihaela PerteaSteven L Salzberg
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Jan 1, 2014·Korean Journal for Food Science of Animal Resources·Young Sik BaeCheorun Jo
Oct 21, 2016·Journal of Animal Physiology and Animal Nutrition·Z Q LuY Z Wang
Dec 28, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Katarzyna Ropka-MolikGrzegorz Żak

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Methods Mentioned

BETA
RNA-seq
PCR
transfection
chip

Software Mentioned

DESeq R
KOBAS
Hisat2
GOseq R
Cufflinks
StringTie
Primer Premier

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