Identification of differentially expressed transcripts and translatants targeted by knock-down of endogenous PCBP1

Biochimica Et Biophysica Acta
Li-Rong HuoNanbert Zhong

Abstract

PCBP1 is a member of the hnRNP family and participates in the regulation of transcription and translation. Previously, we identified transcripts targeted by overexpression of exogenous PCBP1. To further determine if these altered transcripts may also be targeted by a lack of PCBP1, we depleted endogenous PCBP1 in human SH-SY5Y cells. We identified 941 transcripts with the Affymetrix and 1362 with the Agilent expression platforms. There were 375 transcripts identified by both platforms, including 328 down-regulated and 47 up-regulated. The identified transcripts could be grouped into neuronal, cell signaling, metabolic, developmental, and differentiation categories, with pathway involvement in Wnt signaling, TGF beta signaling, translation factors and nuclear receptors. A proteomic profiling study with a two-dimensional chromatographic platform showed global translational changes over a range of isoelectric points (pI)=4.84-8.42. This study identifies the transcripts affected by knock-down of endogenous PCBP1 and compares them to the transcripts affected by overexpression of PCBP1.

References

Nov 1, 2000·Cell·M Bienz, H Clevers
Jun 1, 2002·Science·Liliana Attisano, Jeffrey L Wrana
Dec 20, 2002·Development·Fumi KuboShinichi Nakagawa
May 8, 2004·Trends in Biochemical Sciences·Peter ten Dijke, Caroline S Hill
Sep 24, 2004·Biology of the Cell·Antje Ostareck-Lederer, Dirk H Ostareck
Oct 27, 2005·Biochemical and Biophysical Research Communications·Nanbert ZhongW Ted Brown
Aug 15, 2006·The International Journal of Biochemistry & Cell Biology·Pascal Bernard, Vincent R Harley
Sep 19, 2006·Biochemical and Biophysical Research Communications·Andrea M BerryJane L Ko
Feb 10, 2007·Experimental and Molecular Pathology·Wei-Qing XuJian-Min Tang
Jun 22, 2007·Journal of Virology·Rushika PereraBert L Semler
Sep 1, 2007·Antioxidants & Redox Signaling·Ronald C Wek, Douglas R Cavener
Oct 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chung-Ying HuangPeter S Nelson
Dec 20, 2007·Neural Development·Olivier MautiEsther T Stoeckli
Jan 12, 2008·Molecular Cancer Therapeutics·Silvia PrisleiCristiano Ferlini
Jan 16, 2008·Journal of Neurochemistry·Fang CaiWeihong Song
Jan 23, 2008·Journal of Cancer Research and Clinical Oncology·Teresa A ZimmersLeonidas G Koniaris
May 31, 2008·Science·Haifeng ShiCaroline C Philpott
Jul 29, 2008·Biochimica Et Biophysica Acta·Li-Rong Huo, Nanbert Zhong

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Citations

Jan 1, 2012·The Tohoku Journal of Experimental Medicine·Patrick TaeJoon HwangChan-Wha Kim
Jun 22, 2019·Biochemistry. Biokhimii︠a︡·I B NazarovA N Tomilin
Oct 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xuetian ZhangHong Zhang

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