Identification of differentially expressed microRNAs in placentas of cloned and normally produced calves by Solexa sequencing

Animal Reproduction Science
Jianmin SuYong Zhang

Abstract

Aberrant gene expression caused by aberrant nuclear reprogramming results in developmental abnormalities in cloned animals and ultimately their death. MicroRNAs (miRNAs), a family of ∼ 22 nucleotide, non-coding, single-stranded RNA molecules, are considered as key regulators of gene expression. Numerous miRNAs and their expression patterns have been identified in various species. However, the significance of miRNAs in developmental abnormalities in cloned animals is unclear. Small RNA libraries were generated from the placentas of cloned (somatic cell nuclear transfer, SCNT) and normally produced (control) calves. A total of 18,815,541 clean reads were obtained from the SCNT library and 19,329,352 from the control library. In total, 430 conserved bovine miRNAs were identified in bovine placenta. Furthermore, the family, expression predominance, and base substitution of the conserved miRNAs were also analyzed. We found 135 conserved miRNAs that were differentially expressed significantly between the two samples, which suggest that these miRNAs may affect developmental abnormalities in cloned cattle and ultimately their death. The miRNA target prediction, gene ontology, and pathway analysis for these target genes were also carrie...Continue Reading

References

Dec 16, 1998·Science·Y KatoY Tsunoda
Aug 19, 2000·Science·A OnishiA C Perry
Jun 23, 2001·Biology of Reproduction·P A De SousaI Wilmut
Aug 11, 2001·Science·W M RideoutR Jaenisch
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 23, 2002·Nature·T ShinM Westhusin
Mar 30, 2002·Nature Biotechnology·Patrick ChesnéJean-Paul Renard
Dec 20, 2002·Developmental Biology·Hiroshi SuemizuMinoru S H Ko
May 31, 2003·Science·Gordon L WoodsBarry J Pate
Dec 19, 2003·Genes & Development·Rui YiBryan R Cullen
Sep 17, 2004·Nature·Victor Ambros
Nov 9, 2004·Nature·Ahmet M DenliGregory J Hannon
Aug 5, 2005·Nature·Byeong Chun LeeWoo Suk Hwang
Dec 6, 2005·Developmental Biology·Baohong ZhangTodd A Anderson
Apr 6, 2006·Developmental Biology·Ziyi LiJohn F Engelhardt
Jun 1, 2006·Nature Genetics·Bryan R Cullen
Jul 18, 2006·Nucleic Acids Research·Jan Krüger, Marc Rehmsmeier
Jun 15, 2007·Theriogenology·M A MiglinoA M Carter
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·A RosaI Bozzoni
Jan 30, 2008·Bioinformatics·Ruiqiang LiJun Wang
Feb 19, 2008·Placenta·D R ArnoldL C Smith
Aug 13, 2008·Developmental Cell·Shusheng WangEric N Olson
Aug 13, 2008·Developmental Cell·Jason E FishDeepak Srivastava
Sep 13, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Christelle Borel, Stylianos E Antonarakis
Jan 8, 2009·Biochemical and Biophysical Research Communications·Xiang-Shun CuiNam-Hyung Kim
Mar 4, 2009·Nucleic Acids Research·H Alexander EbhardtRichard P Fahlman
May 9, 2009·Gene·Baohong ZhangXiaoping Pan
Jul 3, 2009·Biology of Reproduction·Steve R BischoffJorge A Piedrahita
Sep 22, 2009·BMC Genomics·Md Munir HossainDawit Tesfaye
Mar 30, 2010·Animal Reproduction Science·Swamy K TripuraniJianbo Yao
Oct 19, 2010·Cell Host & Microbe·Cécilia ChassinMathias W Hornef
Nov 3, 2010·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Dec 22, 2010·BMC Genomics·Yijun MengMing Chen

❮ Previous
Next ❯

Citations

Sep 15, 2016·Reproduction, Fertility, and Development·R PasquarielloJ L Williams
Dec 24, 2018·Reproduction in Domestic Animals = Zuchthygiene·Jun-Li WangJian-Ming Gao
Feb 20, 2020·Stem Cell Research & Therapy·Pengxiang QuEnqi Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

22q11 Deletion Syndrome

22q11.2 deletion syndrome, also known as DiGeorge syndrome, is a congenital disorder caused by a partial deletion of chromosome 22. Symptoms include heart defects, poor immune system function, a cleft palate, complications related to low levels of calcium in the blood, and delayed development. Discover the latest research on this disease here.