Identification of differentially expressed proteins between free-living and activated third-stage larvae of Haemonchus contortus

Veterinary Parasitology
Fang WangRuofeng Yan

Abstract

The disease caused by Haemonchus contortus, a blood-feeding nematode of small ruminants, is of major economic importance worldwide. The infective third-stage larva (L3) of this nematode is enclosed in a second cuticle. Once the L3 is ingested by the host, the outer cuticle undergoes an exsheathment process that marks the transition from the free-living stage to the parasitic stage. This study explored the changes in protein expression relative to this transition. Proteins extracted from free living L3 and exsheathed L3 (xL3) were analyzed by two dimensional differential gel electrophoresis (2D-DIGE). More than 2200 protein spots were recognized, and 124 of them was found to be differentially expressed (average ratio of xL3/L3>1.5 or xL3/L3<-1.5, p<0.05). Of these, 83 spots were up-regulated and 41 spots were down-regulated in xL3 when compared with L3. These differentially expressed spots were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) or MALDI-TOF-MS/MS and 40 proteins were identified. To predict the functions of these identified proteins, they were assigned for gene ontology (GO) annotation. Results showed that the proteins may be involved in biological processes of...Continue Reading

References

Apr 1, 1993·International Journal for Parasitology·E A MunnC A Greenwood
Aug 22, 2001·International Journal for Parasitology·D HartmanS E Newton
Feb 3, 2004·Journal of Chromatography. a·Lei JiangMichael Fountoulakis
Jun 2, 2005·Parasitology Research·T NakadaY Takahashi
Jun 9, 2006·International Journal for Parasitology·S Nikolaou, R B Gasser
Oct 13, 2006·International Journal for Parasitology·Marie LabbéPierre Péry
Apr 6, 2011·Parasitology Research·Lorena Mayana Beserra de OliveiraMayara de Aquino Mesquita
Dec 14, 2011·Veterinary Parasitology·Ray M Kaplan, Anand N Vidyashankar
Sep 23, 2014·Acta Parasitologica·Ruofeng YanXiangrui Li

❮ Previous
Next ❯

Citations

Sep 18, 2016·Molecular and Biochemical Parasitology·Rita Leal SperottoSibele Borsuk
Feb 23, 2019·Parasitology Research·Jinglong ChenXiaojing Yang
Apr 20, 2017·Parasites & Vectors·Baojie LiRuofeng Yan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.