Identification of differentially expressed microRNAs in Sahiwal (Bos indicus) breed of cattle during thermal stress

Cell Stress & Chaperones
G SengarC G Joshi

Abstract

microRNAs (miRNAs) are a class of small non-coding RNAs that play key roles in post transcriptional gene regulation that influence various fundamental cellular processes, including the cellular responses during environmental stresses. However, perusal of literatures revealed few reports on the differential expression of miRNA during thermal stress in Indian native (Bos indicus) cattle breeds. The present investigation aimed to identify differentially expressed miRNAs during thermal stress in Sahiwal (Bos indicus) dairy cattle breed of India, adapted with tropical climate over a long period of time. Stress responses of the animals were characterized by determining various physiological as well as biochemical parameters and differential expression profile of major heat shock protein genes. Ion Torrent deep sequencing and CLC-genomic analysis identified a set of differentially expressed miRNAs during summer and winter seasons. Most of the identified differentially expressed miRNAs were found to target heat shock responsive genes especially members of heat shock protein (HSP) family. Real-time quantification-based analysis of selected miRNAs revealed that bta-mir-1248, bta-mir-2332, bta-mir-2478, and bta-mir-1839 were significantly...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 26, 2003·Genes & Development·John G DoenchPhillip A Sharp
Dec 31, 2003·Cell·Benjamin P LewisChristopher B Burge
Jun 11, 2004·Annual Review of Biochemistry·Aziz SancarStuart Linn
Jan 16, 2007·Nature Genetics·Alex S FlyntJames G Patton
Jul 24, 2007·Nature Genetics·José Manuel Franco-ZorrillaJavier Paz-Ares
Nov 13, 2007·Cancer Cell·Heiko Hermeking
Apr 30, 2008·The Journal of Biological Chemistry·Sebastian Karl WandingerJohannes Buchner
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Aug 25, 2009·Emergency Medicine Journal : EMJ·P Davies, I Maconochie
Oct 23, 2010·Molecular Cell·Anthony K L Leung, Phillip A Sharp
Dec 4, 2010·International Journal of Biometeorology·Worapol AengwanichThongchai Boonsorn
Oct 20, 2011·Tropical Animal Health and Production·Rangsun CharoensookChristoph Knorr
Feb 20, 2014·BMC Genomics·Josue Moura RomaoLe Luo Guan
Aug 8, 2015·Journal of Animal Physiology and Animal Nutrition·I Belhadj SlimenM Abdrrabba
Aug 27, 2015·PloS One·Susumu MuroyaMasayuki Hojito
Feb 19, 2016·Journal of Animal Science and Biotechnology·Susumu MuroyaMasaru Nomura

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Citations

May 24, 2020·Molecular Genetics and Genomics : MGG·Wellison Jarles da Silva DinizLuciana Correia de Almeida Regitano

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Methods Mentioned

BETA
thermal stress
PCR
chip
RNA-Seq
MDA
electrophoresis
environmental stresses
environmental stress

Software Mentioned

SPSS
BLAST
CLC
miRNet
RNA fold
2100
– Genomics - work bench
BLASTn

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