Identification of differently expressed genes in human colorectal adenocarcinoma

World Journal of Gastroenterology : WJG
Yao ChenZeng-Ni Yi

Abstract

To investigate the differently expressed genes in human colorectal adenocarcinoma. The integrated approach for gene expression profiling that couples suppression subtractive hybridization, high-throughput cDNA array, sequencing, bioinformatics analysis, and reverse transcriptase real-time quantitative polymerase chain reaction (PCR) was carried out. A set of cDNA clones including 1260 SSH inserts amplified by PCR was arrayed using robotic printing. The cDNA arrays were hybridized with fluorescent-labeled probes prepared from RNA of human colorectal adenocarcinoma (HCRAC) and normal colorectal tissues. A total of 86 genes were identified, 16 unknown genes and 70 known genes. The transcription factor Somult9 influencing cell differentiation was downregulated. At the same time, Heat shock protein 10 KDis downregulated and Calmoulin is up-regulated. Downregulation of heat shock protein 10 KD lost its inhibition of Ras, and then attenuated the Ras GTPase signaling pathway, increased cell proliferation and inhibited cell apoptosis. Down-regulated transcription factor Somult9 influences cell differentiation and cell-specific gene expression. Down-regulated Somult9 also decreases its binding to calmodulin, accumulates calmodulin as rec...Continue Reading

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Citations

Jun 14, 2012·Epigenomics·Ali R JazirehiPeter B Wenn
Oct 2, 2012·Oncology Reports·Jianjun ZhuYao Chen
Feb 14, 2013·Molecular Medicine Reports·Xiaoxia WenYao Chen
Jun 20, 2013·Molecular Medicine Reports·Kaicheng Wang, Yao Chen
Feb 14, 2016·Expert Opinion on Therapeutic Targets·Ruth NussinovOzlem Keskin
Mar 15, 2012·Journal of Molecular Neuroscience : MN·Ying KongKe-jun Nan
Jul 16, 2014·Medical Oncology·Xiaoxia WenYao Chen
Feb 15, 2012·Virchows Archiv : an International Journal of Pathology·Minhua SunMasashi Fukayama
Mar 22, 2014·Biomedical Reports·Xiaoxia Wen, Yao Chen
Jun 19, 2015·Molecular Cancer Research : MCR·Ruth NussinovOzlem Keskin

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